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| 一种新型植物动力学荧光仪 | |
| 引用本文： | 高荣孚,温晓刚,林世青,林桂康.一种新型植物动力学荧光仪[J].生物物理学报,2000,16(1):183-186. |
| 作者姓名： | 高荣孚 温晓刚 林世青 林桂康 |
| 作者单位： | 1. 北京林业大学,北京100083 2. 中国科学院植物研究所光合作用研究中心,北京100093 3. 北京市圣百灵计算机研究所,北京100083 |
| 基金项目： | 本研究得到中国科学院以及国家基金委(编号:39670068,39800086)、北京市基金委(编号:6972012)的部分资助, |
| 摘 要： | 当植物从暗中转到光下时 ,其体内叶绿素 (Ｃｈｌ)荧光强度会随照光时间产生有规律的变化 ,这就是植物荧光诱导现象。由于它能够灵敏、快速、简便和无损伤地探测植物体内光合生理状况及环境因子对植物的影响 ,近年来在植物生理、植物生态、农业、林业、环境污染和遥感等领域得到重视和应用［１，２，３］。植物动力学荧光仪有调制式和非调制式两类 ,非调制式荧光仪特别适合于荧光诱导上升曲线及曲线中偏转荧光 (ＦＩ)和荧光上升互补面积 (ＣＡ)的研究 ,其中ＦＩ 是快速简便地探测体内ＰＳＩＩ无活性中心相对含量的重要途径 ,后者与光合激发能… |
| 关 键 词： | 植物动力学荧光仪 植物产量荧光仪 设计 性能 |
| 文章编号： | 1000-6737(2000)01-0183-04 |
| 修稿时间： | 1999年7月19日 |
A NEW TYPE OF THE PLANT KINETICS FLUOROMETER |
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| GAO Rongfu,WEN Xiaogang,LIN Shiqing,LIN Guikang.A NEW TYPE OF THE PLANT KINETICS FLUOROMETER[J].Acta Biophysica Sinica,2000,16(1):183-186. | |
| Authors: | GAO Rongfu WEN Xiaogang LIN Shiqing LIN Guikang |
| Institution: | GAO Rongfu1,WEN Xiaogang2,LIN Shiqing2,LIN Guikang3 |
| Abstract: | A new type of home-made computer-controlled non-modulated chlorophyll a fluorometer, the Plant Productivity Fluorometer PFM-101, is introduced including its main features, design and some applications. By using a latest highly integrated PIN-photodiode and FET linear amplifier in the PFM-101 the fluorescence kinetics curves and various fluorescence parameters can be measured and calculated automatically. It has superiority over the commercialized foreign ones in its versatile functions, super-low power consumption, high accuracy, low zero drift, low background noise and high stability etc. The errors produced in the measuring process are reduced to minimum by a unique data processing. Especially, the constant (Fo) and the inflection fluorescence (FI) can be determined with great precision and the inactive PS II reaction center can be calculated automatically. The instrument can be widely used in the field of agriculture, forestry, plant physiology and plant ecology, etc. |
| Keywords: | Chlorophyll Fluorescence kinetics Fluorometer Design |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |