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1.
Intact plants and isolated leaves of Zostera capricornii Martens ex Aschers were transferred from daylight to darkness. Substantial amounts of chloropyll a and b continued to accumulate in immature and mature tissue in the same ratio as in the light and were incorporated into chlorophyll-protein complexes in the thylakoids. A small amount of protochlorophyllide also accumulated in immature tissue in the dark. Proplastids and immature chloroplasts continued to develop into mature chloroplasts in the dark in the normal manner but prolamellar bodies, which are a conspicuous feature of immature chloroplasts, took longer to disperse than in the light. Protochlorophyllide accumulation and prolamellar-body formation were not correlated. The results indicate that Zostera has a genetic capacity for dark chlorophyll synthesis which is expressed in immature and mature leaf tissue and enables this plant to continue synthesising chlorophyll and assembling chloroplasts at night.Abbreviations Chl
chlorophyll
-
T
o
time of transfer to darkness 相似文献
2.
Samples of microphytobenthos from the Tagus estuary were analysed for photosynthetic pigments by spectrophotometry and High
Performance Liquid Chromatography (HPLC). Chlorophyll a values obtained with HPLC and spectrophotometry methods presented a highly significant positive correlation for both spectrophotometric
methods used (with and without the correction for pheopigments), but this relationship depended on the type of sediment. We
concluded that spectrophotometric methods give reliable Chl-a values, being suited for routine analysis, when a vast number of replicates is needed. However, for the correct estimation
of pheopigments, HPLC analysis is indispensable. In the literature, Chl-a estimations are expressed per content (μg g−1) or concentration (mg m−2). We discuss the influence of sediment type on the results depending on the type of unit used, and propose a simple conversion
factor based on sediment water content. 相似文献
3.
《Fungal biology》2020,124(10):903-913
Although water is essential for photosynthetic activation in lichens, rates of vapor uptake and activation in humid air, which likely influence their niche preferences and distribution ranges, are insufficiently known. This study simultaneously quantifies rehydration kinetics and PSII reactivation in sympatric, yet morphologically and functionally distinct cephalolichens (Lobaria amplissima, Lobaria pulmonaria, Lobaria virens). High-temporal resolution monitoring of rehydrating thalli by automatic weighing combined with chlorophyll fluorescence imaging of maximal PSII efficiency (FV/FM) was applied to determine species-specific rates of vapor uptake and photosynthetic activation. The thin and loosely attached growth form of L. pulmonaria rehydrates and reactivates faster in humid air than the thick L. amplissima, with L. virens in between. This flexible hydration strategy is consistent with L. pulmonaria’s wide geographical distribution stretching from rainforests to continental forests. By contrast, the thick and resupinate L. amplissima reactivates slowly in humid air but stores much water when provided in abundance. This prolongs active periods after rain, which could represent an advantage where abundant rain and stem flow alternates with long-lasting drying. Understanding links between morphological traits and functional responses, and their ecological implications for species at risk, is crucial to conservation planning and for modelling populations under various climate scenarios. 相似文献
4.
In the lab, we exposed three foliose lichen species, Lobaria pulmonaria, Parmelia sulcata and Xanthoria aureola, to 0, 0.01, 0.2, and 0.6 M NaCl in combinations with copper and zinc (0, 10, 100, 500 μM). High salt concentrations adversely affected the lichen membrane integrity as measured by conductivity methods, whereas the potential photosystem II efficiency (Fv/Fm) was tolerant. High light was necessary to reduce Fv/Fm in thalli exposed to salt, whereas high light did not aggravate the conductivity. The seashore species X. aureola was much more resistant to salt than the old forest species L. pulmonaria. With respect to Cu and Zn, used concentrations had no (P. sulcata, X. aureola) or small (L. pulmonaria) effects on Fv/Fm. However, both heavy metals substantially increased conductivity in all species, consistent with membrane damage. Thus, the conductivity method detected high salt, high copper and high zinc stress much more efficiently than did the chlorophyll fluorescence method. This suggests that membrane integrity of the mycobiont is more sensitive to salt and heavy metal stress than potential photosystem II efficiency of its autotrophic partners. 相似文献
5.
An analysis of the photo-induced decline in the in vivo chlorophyll a fluorescence emission (Kautsky phenomenon) from the bean leaf is presented. The redox state of PS II electron acceptors and the fluorescence emission from PS I and PS II were monitored during quenching of fluorescence from the maximum level at P to the steady state level at T. Simultaneous measurement of the kinetics of fluorescence emission associated with PS I and PS II indicated that the ratio of PS I/PS II emission changed in an antiparallel fashion to PS II emission throughout the induction curve. Estimation of the redox state of PS II electron acceptors at given points during P to T quenching was made by exposing the leaf to additional excitation irradiation and determining the amount of variable PS II fluorescence generated. An inverse relationship was found between the proportion of PS II electron acceptors in the oxidised state and PS II fluorescence emission. The interrelationships between the redox state of PS II electron acceptors and fluorescence emission from PS I and PS II remained similar when the shape of the induction curve from P to T was modified by increasing the excitation photon flux density. The contributions of photochemical and non-photochemical quenching to the in vivo fluorescence decline from P to T are discussed. 相似文献
6.
A spontaneously occurring chloroplast genome (plastome) mutant of Oenothera , IVβ, was identified as a single point mutation in the Rubisco large subunit gene (G337 → C), leading to an V113L exchange, which topologically occurs at the interface of two adjacent large subunits (LSU). The minor sterical hindrance of dimer formation by this amino acid exchange strongly impairs holoenzyme assembly, leading to an accumulation of a processing precursor of the holoenzyme, the B‐complex, consisting of one LSU and 14 units of chaperonine 60 (cpn60). It is associated with very low holoenzyme concentrations in the mutant tissue, but does not affect the kinetic properties of the enzyme once assembled. When grown under moderate or low light, leaf tissue containing the plastome mutant showed decreased Chl contents and Chl a / b ratios, increased relative carotenoid contents and violaxanthin deepoxidation activity, but very low CO2 fixation and O2 evolution rates and was very sensitive to photoinhibition. The light dependence of chlorophyll fluorescence quenching components at low temperature resembled an extremely chilling sensitive Oenothera genotype as compared to the wild‐type. The IVβ mutant thus behaves similarly to the Rubisco SSU antisense plants analysed by Stitt and co‐workers (summarised by Stitt and Schulze 1994) and gives an example of the possible influence of plastome mutations on the sensitivity of the photosynthetic apparatus to excess light by modifying the capacity of the Calvin cycle. 相似文献
7.
《Harmful algae》2016
Using satellite imagery to quantify the spatial patterns of cyanobacterial toxins has several challenges. These challenges include the need for surrogate pigments – since cyanotoxins cannot be directly detected by remote sensing, the variability in the relationship between the pigments and cyanotoxins – especially microcystins (MC), and the lack of standardization of the various measurement methods. A dual-model strategy can provide an approach to address these challenges. One model uses either chlorophyll-a (Chl-a) or phycocyanin (PC) collected in situ as a surrogate to estimate the MC concentration. The other uses a remote sensing algorithm to estimate the concentration of the surrogate pigment. Where blooms are mixtures of cyanobacteria and eukaryotic algae, PC should be the preferred surrogate to Chl-a. Where cyanobacteria dominate, Chl-a is a better surrogate than PC for remote sensing. Phycocyanin is less sensitive to detection by optical remote sensing, it is less frequently measured, PC laboratory methods are still not standardized, and PC has greater intracellular variability. Either pigment should not be presumed to have a fixed relationship with MC for any water body. The MC-pigment relationship can be valid over weeks, but have considerable intra- and inter-annual variability due to changes in the amount of MC produced relative to cyanobacterial biomass. To detect pigments by satellite, three classes of algorithms (analytic, semi-analytic, and derivative) have been used. Analytical and semi-analytical algorithms are more sensitive but less robust than derivatives because they depend on accurate atmospheric correction; as a result derivatives are more commonly used. Derivatives can estimate Chl-a concentration, and research suggests they can detect and possibly quantify PC. Derivative algorithms, however, need to be standardized in order to evaluate the reproducibility of parameterizations between lakes. A strategy for producing useful estimates of microcystins from cyanobacterial biomass is described, provided cyanotoxin variability is addressed. 相似文献
8.
HANS RYBERG LENNART AXELSSON KARL-OLOF WIDELL HEMMING I. VIRGIN 《Physiologia plantarum》1980,49(4):431-436
Abstract When dark grown leaves of wheat (Triticum aesivum L.) were given a brief irradiation, there was an immediate onset of chlorophyll (Chl) b synthesis in the dark. This synthesis led to a rather slow accumulation of Chl b, which ceased when the Chl b/Chl a ratio had reached a value of about 0.1. The Chl b synthesis occurred also when the seedlings were treated with the herbicide SAN 9789. Leaves grown under different intensities of red light accumulated Chl b and Chl a, resulting in a ratio Chl b/Chl a which depended on the light intensity. If the light intensity was low, Chl a accumulated to a level about ten times the level of PChlide of the dark grown leaves. This occurred without any increase in the Chl b/Chl a ratio. There was no difference between SAN 9789-treated seedlings and water controls in this respect. Above a certain threshold of irradiance, the Chl b/Chl a ratio in the control leaves increased rapidly with the irradiation intensity. The increase in Chl b/Chl a ratio coincided with formation of grana in the plastids. This increase was not found and grana formation was completely absent in the seedlings treated with SAN 9789. The possibility of two different stages in the Chl b synthesis is discussed. 相似文献
9.
Valérian Meline Wesley Delage Chrystelle Brin Camille Li-Marchetti Daniel Sochard Matthieu Arlat Céline Rousseau Armelle Darrasse Martial Briand Guillaume Lebreton Perrine Portier Marion Fischer-Le Saux Karine Durand Marie-Agnès Jacques Etienne Belin Tristan Boureau 《Molecular Plant Pathology》2019,20(1):33-50
Cases of emergence of novel plant-pathogenic strains are regularly reported that reduce the yields of crops and trees. However, the molecular mechanisms underlying such emergence are still poorly understood. The acquisition by environmental non-pathogenic strains of novel virulence genes by horizontal gene transfer has been suggested as a driver for the emergence of novel pathogenic strains. In this study, we tested such an hypothesis by transferring a plasmid encoding the type 3 secretion system (T3SS) and four associated type 3 secreted proteins (T3SPs) to the non-pathogenic strains of Xanthomonas CFBP 7698 and CFBP 7700, which lack genes encoding T3SS and any previously known T3SPs. The resulting strains were phenotyped on Nicotiana benthamiana using chlorophyll fluorescence imaging and image analysis. Wild-type, non-pathogenic strains induced a hypersensitive response (HR)-like necrosis, whereas strains complemented with T3SS and T3SPs suppressed this response. Such suppression depends on a functional T3SS. Amongst the T3SPs encoded on the plasmid, Hpa2, Hpa1 and, to a lesser extent, XopF1 collectively participate in suppression. Monitoring of the population sizes in planta showed that the sole acquisition of a functional T3SS by non-pathogenic strains impairs growth inside leaf tissues. These results provide functional evidence that the acquisition via horizontal gene transfer of a T3SS and four T3SPs by environmental non-pathogenic strains is not sufficient to make strains pathogenic. In the absence of a canonical effector, the sole acquisition of a T3SS seems to be counter-selective, and further acquisition of type 3 effectors is probably needed to allow the emergence of novel pathogenic strains. 相似文献
10.