大豆异黄酮对雌鼠初情期等生殖性状的影响

目的通过研究大豆异黄酮对雌鼠初情期等生殖性状的影响,为母鼠的安全饲喂提供实验依据。方法本实验选用210只21日龄ICR雌鼠随机分成3组,饲喂含不同浓度大豆异黄酮（0 mg/kg、50 mg/kg、400 mg/kg）的饲料。每日检查母鼠阴道开张和阴道栓情况。采集母鼠45和65日龄时血清,利用ELISA方法检测血清中雌激素含量,并统计45、65日龄体重和子宫重。利用免疫组织化学方法检测子宫上皮雌激素受体的表达分布情况。结果雌鼠阴道开张的平均日龄分别为27.2 d、26.1 d和25.8 d,400 mg/kg组显著早于剂量为0mg/kg的对照组（P〈0.05）;雌鼠平均初次配种时间各组间差异不显著（P〉0.05）;饲养到45日龄和65日龄时400 mg/kg组雌鼠体重显著高于对照组（P〈0.05）。喂养8周后两个试验组在子宫间质细胞上雌激素受体的阳性细胞表达率显著高于对照组;在腺上皮中400 mg/kg组显著高于50 mg/kg组和对照组（0 mg/kg）;但是在腔上皮中,各组间受体的阳性细胞表达率差异不显著（P〉0.05）。结论饲喂每公斤含400 mg大豆异黄酮的饲料可以刺激初情期前母鼠阴道的开张,提高雌鼠的体重,降低65日龄时血清雌激素含量,并在饲喂8周后影响子宫间质和腺上皮上雌激素受体的表达分布;而饲喂每公斤含50 mg大豆异黄酮的饲料仅对小鼠45日龄体重及饲喂8周后子宫间质上雌激素受体的表达分布有所影响。

Effect of Soy Isoflavones on Initial Oestrus and Some Reproductive Physiological Characteristics of Female Mice

Objective This study is aimed to investigate the effect of soy isoflavones on initial oestrus and some physiological characteristics of female mice.Methods Total two hundred and ten 3-week old female ICR mice were randomly divided into 3 groups,and fed with the control（0 mg/kg）,low soy isoflavone level（50 mg/kg） and high soy isoflavone level（400 mg/kg） diet separately.The state of vagina opening and vagina plug after treatment with soy isofalvones,the concentration of estrogen in their serum on Day45 and Day65 as well as their body,uterus and ovarian weight,were analyzed and recorded separately.The ER（estrogen receptor） on uterus was detected by immunohistochemical staining.Results There were significant differences of the durations for murine vagina opening between those treatment groups and control one.The body weight of two treatment groups was significantly lower than that of the control one.The positive rate of ER on the stroma in control group was significantly lower than that of the other treatment ones,but that on the glandular epithelium in high level group was much higher than the other groups＇,and there were no significant differences on the luminal epithelium among all the groups.Conclusions Collectively,it can be concluded that the 400mg/kg soy isoflavones in the diet can simulate murine vagina opening,increase their body weight and degrade the estradiol level in their serum on Day65.affect the expression of estrogen receptor on their utrine stroma,luminal epithetlium after 8 weeks.however,50mg/kg soy isoflavones in the diet only affect the body weight of 45d,and the expression of estrogen receptor on their utrine stroma after 8 weeks.