结核分枝杆菌感染小鼠的脾脏和肺脏组织荷菌量与病理变化

目的分析结核急性感染小鼠脾脏和肺脏的组织荷菌量以及病理的动态变化。方法以3×105CFU结核分枝杆菌标准株H37Rv尾静脉途径感染雌性C57BL/6J小鼠,测定感染后1 d、1周、2周、3周、4周、6周和8周肺脏及脾脏组织的荷菌量,脾脏和肺脏组织经HE染色分析病理变化。结果感染动物的脾脏荷菌量在前3周逐步提高,在4～8周脾脏荷菌量维持稳定,肺脏组织在2～8周组织荷菌量逐步升高。病理分析显示动物感染3周后肺脏和脾脏出现肉芽肿,在6～8周病理损伤加重。结论小鼠经尾静脉感染高剂量结核分枝杆菌在8周前表现为急性感染状态,适用于抗结核药物的体内药效学评价。

Bacterial Load and Pathological Changes in the Spleen and Lung of M. tuberculosis Hematogenous Infected Mice

Objective To establish a mouse model of acute infection with M.tuberculosis suitable for pharmacodynamic evaluation of anti-TB drug candidates.Methods Female C57BL/6J mice were infected with intravenous injection of 3×105 CFU M.tuberculosis H37Rv.Bacterial load and pathological changes in the spleen and lung were analyzed at 1 d,1 w,2 w,3 w,4 w,6 w and 8 w post-infection.Results The bacteria load in the spleen in increased during the first 3 weeks,and maintained at a high level during the following 5 weeks.The bacteria load in the lung increased during the following 8 weeks after infection.Granuloma formation could be detected in the lung and spleen at 3 weeks after infection,and pathological injury became more severe at 6-8 weeks.Conclusions Mice hematogenously infected with a high dose of M.tuberculosis show an acute infectious condition.The mouse model of acute TB infectioon can be used in evaluation of anti-TB drug candidates.