| 红鲫C1HD近交系RAPD标记的建立 |
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| 引用本文： | 田智,;吴端生,;郑济芳,;江千秋.红鲫C1HD近交系RAPD标记的建立[J].中国实验动物学杂志,2007(10):589-591. |
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| 作者姓名： | 田智 ;吴端生 ;郑济芳 ;江千秋 |
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| 作者单位： | [1]南华大学生命科学与技术学院; [2]南华大学实验动物学部,湖南衡阳421001; [3]长沙医学院,长沙410219 |
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| 基金项目： | 湖南省自然科学基金项目资助（N0.04JJ30046）. |
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| 摘 要： | 目的建立红鲫C1HD近交系的RAPD标记。方法从80条随机引物中筛选出20条扩增效果和多态性较好的引物,对8尾红鲫C1HD近交系和8尾普通红鲫基因组DNA进行RAPD扩增。结果S333引物扩增出一条特异性条带,大小约为2.1 kb。结论S333引物扩增出的特异性条带可以作为区分普通红鲫与红鲫C1HD近交系的分子遗传标记。
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| 关 键 词： | 近交系 红鲫 分子标记 RAPD |
Bolting of RAPD Marker on C1HD Inbred Red Crucian Carp,Carrassius auratus Red Variety |
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| Institution: | TIAN Zhi , WU Duan-sheng,ZHEN Ji-fang , JIANG Qian-qiu ( 1. College of Life Science and Technology of University of South China; 2. Department of Laboratory Animal Science of University of South China, Hengyang 421001, China; 3 .Changsha Medical College, Changsha 410219, China) |
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| Abstract: | Objective To bolt RAPD marker from Carassius auratus red variety inbred strain CIHD. Methods Using 20 random primers from 80 primers, 8 samples of inbred strain C1HD and common Carassius auratus red variety were analyzed by random amplified polymorphic DNA （RAPD）, respectively. Results A primer （$333） was observed to produce a specific band. Conclusion This band could be used as a molecular marker for discriminating Carassius auratus red variety inbred strain CIHD from common population. |
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| Keywords: | Inbred strain Carassius auratus red variety Molecular marker RAPD |
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