利用RAPD 标记技术对白桦种源遗传变异的分析及种源区划

用随机扩增多态DNA(RAPD)分子标记方法对白桦17 个种源152 个个体进行了遗传变异的比较分析, 通过14 个随机引物扩增共检测到233 个位点, 各种源多态位点百分率差异明显, 范围在20.17%~32.19%之间, 多态位点百分率最高的是帽儿山种源和清源种源, 最低的是绰尔种源。遗传变异在种源间占43.53%, 在种源内个体间占56.47%。根据种源间的遗传距离, 构建了白桦17 个种源的遗传关系聚类图, 结果将东北地区的白桦聚为一类, 华北、西北地区的白桦聚为另一类。同时根据地理气候因子和遗传距离对白桦群体进行了种源区的划分。

ANALYSIS OF GENETIC VARIATION WITHIN AND AMONG BETULA PLATYPHYLLA PROVENANCES AND PROVENANCE DIVISION USING RAPD MARKERS

RAPD was used to detect genetic variation among 17 provenances, including 152 individuals. 233 loci were amplified using 14 random primers. The differentiation of the percentage of polymorphic loci among different provenances was evident, ranging from 20.17% to 32.19%, among which there were Maoershan provenance and Qingyuan provenance with the highest PPL and Chaoer provenance with the lowest PPL. Most of the variation (56.47%) occurred among individuals within provenances, and 43.53% was attributed to differences among provenances. The dendrogram of genetic relationship was constructed according to genetic distances among provenances. The result revealed that the provenances from Northeast and others from North and Northwest were respectively clustered into different groups. At the same time, provenance division was completed on the basis of geographic climatic factors and genetic distances.