二色胡枝子遗传多样性AFLP分析

利用AFLP对二色胡枝子14个居群的161个个体的遗传多样性进行分析，5对引物共扩增出253条带，多态带百分率（P）为96.8%，二色胡枝子种群的平均位点等位基因数（Ao）为1.968，平均有效等位基因数（Ae）为1.643，Nei’s基因多样性指数（H）为0.369，Shannon信息指数（I）为0.544。14个种源的平均多态性条带数为159条，平均多态带百分率为62.9%，平均Nei’s基因多样性指数为0.257，I＝0.373。二色胡枝子遗传多样性的76.68%分布于种源内（Φst＝0.233 2），各种源间的差异显著。聚类结果表明，二色胡枝14个种源可划分为三个大类，居群的遗传多样性参数与其地理、生态因子均不显著相关，遗传多样性无明显地域分布格局。

AFLP Analysis of Genetic Diversity Among Lespedeza bicolor Populations

Genetic diversities of Lespedeza bicolor were evaluated using AFLP in this study. Five pairs of primers were used for evaluating genetic diversity. 253 AFLP markers were observed,among which 245 markers (PPL=96.8%) were polymorphic. At species level,Ao=1.968,Ae=1.643,H=0.369 and I=0.544. And at population level,P=62.99%,Ao=1.605,Ae=1.479,H=0.257,I=0.373. The coefficient of genetic differentiation was 0.233 2, which indicated that 76.68% variability occurred in populations of L.bicolor 14 populations of L.bicolor were divided into three groups by the dendrogram constructed using Nei’s genetic distance. There was no relativity between the genetic diversity parameters (P,H,I) and geographic &; climatic factors (longitude,latitude,AMT,AMP), and no fixed distribution pattern of genetic diversity was found either.