中国东北落叶松属植物rbcL 基因的序列分析及系统演化

长白落叶松的分类位置及与兴安落叶松的种间关系, 长期以来未有定论。本研究从大兴安岭、小兴安岭及张广才岭等6 个地点采集实验材料, 对其叶绿体DNA 的rbcL 基因进行PCR-RFLP 分析并测序。结果显示它们的rbcL 基因序列均没有差异, 表明东北地区落叶松属植物有共同起源。在系统演化上, 长白落叶松来自兴安落叶松(Larix gmelinii Rupr.), 应作为兴安落叶松的变种即L.gmelinii Rupr.var.olgensis Ostenfeld et S.Larsen

GENE SEQUENCE ANALYSIS OF rbcL AND SYSTEMIC EVOLUTION IN LARIX NORTHEAST CHINA

There is no specific conclusion about systematic status of Larix olgensis and species relationship with Larix gmelinii. Experiment materials were taken from six sites including Daxingan Mountain, Xiaoxingan Mountain and Zhangguangcai Mountain. CpDNA rbcL gene of materials is analysized by PCR-RFLP and DNA sequence methods. The results suggest their rbcL gene sequences are the same and plants of Larix in Northeast China have same origin. Larix olgensis evolved from Larix gmelinii in systematic evolution, so it is suitable to regard Larix olgensis as a variety of Larix gmelinii and the name should be Larix gmelinii (Rupr.) Rupr. var. olgensis (Henry) Ostenfeld et S. Larsen.