| 甜菜M14品系花期cDNA文库的构建及特异表达基因的筛选 |
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| 引用本文： | 马春泉,张莹,崔颖,王冰,王玉婷,刘金玲,王宏建,李海英.甜菜M14品系花期cDNA文库的构建及特异表达基因的筛选[J].植物研究,2008,28(4):408-411. |
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| 作者姓名： | 马春泉 张莹 崔颖 王冰 王玉婷 刘金玲 王宏建 李海英 |
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| 作者单位： | 黑龙江大学生命科学学院分子生物学实验室,哈尔滨,150080 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家转基因植物与产业化研究专项资助项目，黑龙江省自然科学基金重点项目，黑龙江大学青年科学基金 |
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| 摘 要： | 构建了甜菜M14品系在花期的ZAP表达载体的cDNA文库，利用抑制消减杂交方法所获得的M14品系特异表达的2个EST片段为探针筛选cDNA文库，获得了M14品系特异表达cDNA片段Me-86和Me-84；进一步利用RACE技术获得了基因M14-86 cDNA片段的全长序列。生物信息学分析表明M14品系特异表达基因M14-86的cDNA片段与cDNA片段Me-84分别与大花马齿苋(Portulaca grandiflora)及瓶子草(Sarracenia purpurea)的26S核糖体RNA具有很高的同源性。
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| 关 键 词： | cDNA文库 EST 表达载体 噬菌斑原位杂交 RACE技术 |
Construction of cDNA Library in Florescence and Screening Specifically Expressed Genes in Sugar Beet M14 |
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| MA Chun-Quan,ZHANG Ying,CUI Ying,WANG Bing,WANG Yu-Ting,LIU Jin-Ling,WANG Hong-Jian,LI Hai-Ying.Construction of cDNA Library in Florescence and Screening Specifically Expressed Genes in Sugar Beet M14[J].Bulletin of Botanical Research,2008,28(4):408-411. |
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| Authors: | MA Chun-Quan ZHANG Ying CUI Ying WANG Bing WANG Yu-Ting LIU Jin-Ling WANG Hong-Jian LI Hai-Ying |
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| Institution: | (Bio-Molecular Lab of College of Life Sciences，Heilongjiang University，Harbin 150080) |
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| Abstract: | The cDNA library of sugar beet M14 in florescence was constructed using ZAP expression vector. Two ESTs, which were specifically expressed in M14 and obtained by suppression subtractive hybridization, were used as probes to screen the cDNA library. Then, two cDNA fragments Me-84 and Me-86 specifically expressed in M14 were obtained. Full length cDNA sequence of gene M14-86 was later obtained using the method of RACE. Bioinformatic analysis of M14-86 and Me-84 cDNA fragments showed high homology with the 26S ribosomal RNA gene in Sarracenia purpurea and Portulaca grandiflora, respectively. |
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| Keywords: | cDNA library EST expression vector plaques in situ hybridization method of RACE |
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