| VEGF、VEGFR2在青春期大鼠睾丸、附睾及附睾精子上的表达 |
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| 引用本文： | 马莉,田宏,张洁,艾庆燕,苗乃周,王海旭,王丽蓉,邱曙东,张秋养.VEGF、VEGFR2在青春期大鼠睾丸、附睾及附睾精子上的表达[J].中国组织化学与细胞化学杂志,2010,19(2):147-151. |
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| 作者姓名： | 马莉 田宏 张洁 艾庆燕 苗乃周 王海旭 王丽蓉 邱曙东 张秋养 |
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| 作者单位： | 1. 西安交通大学医学院人体解剖与组织胚胎学系,陕西,西安,710061
2. 延安大学医学院组织胚胎学教研室,陕西,延安,716000 |
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| 基金项目： | 国家自然科学基金，教育部科学技术重点研究资助项目 |
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| 摘 要： | 目的通过对血管内皮生长因子(VEGF)及其受体VEGFR2在青春期大鼠睾丸及附睾表达的研究,探讨其在雄性生殖器官中的作用。方法采用免疫组化法检测VEGF、VEGFR2在SD大鼠睾丸和附睾的表达定位,用免疫荧光法检测它们在大鼠附睾精子上的表达定位。结果VEGF及VEGFR2在青春期大鼠睾丸和附睾组织中均有表达。在睾丸中,VEGF主要表达于精原细胞胞质、精子细胞发育中的顶体、Sertoli细胞胞质及精子残余体内,Leydig细胞胞质也有阳性表达;VEGFR2主要表达于精子细胞发育中的顶体和间质细胞胞质。在附睾中,VEGF表达于附睾管上皮所有主细胞胞质内;而VEGFR2表达于附睾管头段和尾段上皮主细胞胞质内,体段免疫染色阴性。免疫荧光显示,VEGF与VEGFR2都与精子头部顶体、尾部颈段、中段和主段相结合,末段未见阳性荧光。结论VEGF及VEGFR2在大鼠的睾丸和附睾中均有表达,其表达定位具有细胞特异性和区域特异性,提示其可能在大鼠睾丸精子发生和附睾精子成熟中发挥重要作用。
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| 关 键 词： | 血管内皮生长因子 血管内皮生长因子受体2 睾丸 附睾 精子 免疫组化 免疫荧光 |
EXPRESSIONS OF VEGF AND VEGFR2 PROTEINS IN THE TESTIS,EPIDIDYMIS AND SPERMATOZOA OF ADOLESCENT RATS |
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| Ma Li ,Tian Hong,Zhang Jie,AI Qingyan ,Miao Naizhou,Wang Haixu,Wang Lirong,Qiu Shudong,Zhang Qiuyang.EXPRESSIONS OF VEGF AND VEGFR2 PROTEINS IN THE TESTIS,EPIDIDYMIS AND SPERMATOZOA OF ADOLESCENT RATS[J].Chinese Journal of Histochemistry and Cytochemistry,2010,19(2):147-151. |
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| Authors: | Ma Li Tian Hong Zhang Jie AI Qingyan Miao Naizhou Wang Haixu Wang Lirong Qiu Shudong Zhang Qiuyang |
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| Institution: | Ma Li 2,Tian Hong1,Zhang Jie1,AI Qingyan 2,Miao Naizhou2,Wang Haixu1,Wang Lirong1,Qiu Shudong1,Zhang Qiuyang1 1Department of Human Anatomy and Histo-Embryology,Medical School of Xi'an Jiaotong University,Xi'an 710061,China,2Department of Human Histology and Embryology,Medical School of Yan'an University,Yan'an 716000 |
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| Abstract: | Objective To study the expression of vascular endothelial gro-wth factor(VEGF) and its receptor VEGFR2 in the testis and epididymis of adolescent rats,and to approach the function of both proteins in the male reproductive system.Methods The expressions of VEGF and VEGFR2 were detected in SD rats.Immunohistochemical staining was used for both testis and epididymis and immunofluorescent staining was used for spermatozoa.Results The VEGF and VEGFR2 proteins were presented in the rat testis and epididymis by im... |
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| Keywords: | Vascular endothelial growth factor(VEGF) VEGFR2 Testis Epididymis Spermatozoa Immunohistochemistry Immunofluorescence |
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