实验性肝硬化酶组织化学变化的研究

实验选用Ｗｉｓｔａｒ大鼠１５只，随机分为正常组５只，实验组（４０％ＣＣｌ４植物油皮下注射组）１０只，同时喂养９周。断头法快速处死动物，立即取肝，冰冻切片，用酶组织化学和图像分析法观察肝内主要代谢酶活性变化。结果显示：实验组肝内ＳＤＨ、ＣＣＯ活性降低，ＬＤＨ、ＮＯＳ活性升高，ＭＡＯ活性无明显变化

STUDY ON ENZYME HISTOCHEMISTRY OF EXPERIMENTAL CIRRHOSIS LIVER

15 Wistar rats were divided into 2 groups: including normal control group 5 and experimental Group 10 (subcutaneous injection of CCl 4 diluted by plant oil). The rats were fed under same condition for 9 weeks, then were killed respectively. Using exzyme histochemistry and imaging analysis the actioity changes of major enzymes were observed. The results showed that the activities of SDH, CCO were decreasing while those of LDH, NOS were increasing in chronic cirrhosis model group. The activity changes of MAO were not obvious.