黑龙江林蛙冬眠和非冬眠消化道内分泌细胞的比较研究

目的应用5-羟色胺(5-HT)、生长抑素(SS)、胃泌素(GAS)、胰高血糖素(Glu)、胰多肽(PP)和P-物质(SP)6种特异性抗血清,对黑龙江林蛙冬眠期和非冬眠期消化道内分泌细胞进行检测。方法应用卵白素-生物素-过氧化物酶复合物(avidin-biotin-peroxidase complex,ABC)免疫组织化学法。结果在冬眠期和非冬眠期黑龙江林蛙消化道内检测到5-HT细胞、SS细胞和Glu细胞,而GAS细胞、PP细胞和SP细胞均未检测到。5-HT细胞分布广泛,存在于黑龙江林蛙冬眠期和非冬眠期从食管到直肠的整个消化道中,在冬眠期分布密度高峰为胃幽门部和十二指肠,而非冬眠期则为胃幽门部;SS细胞分布也较为广泛,在黑龙江林蛙冬眠期和非冬眠期消化道中,除了直肠以外,均有分布,且这两个时期SS细胞的分布密度高峰均在胃幽门部。Glu细胞在冬眠期仅在胃贲门部、胃体、空肠和回肠检测到,而在非冬眠期除以上四个部位外直肠部也检测到了Glu细胞,并且Glu细胞分布密度高峰都在胃体部。结论黑龙江林蛙在冬眠期和非冬眠期消化道中内分泌细胞分布型的不同,可能与机体适应不同时期的生理功能有关。

COMPARATIVE RESEARCH OF ENDOCRINE CELLS IN THE DIGESTIVE TRACT OF RANA AMURENSIS DURING HIBERNATION AND NON-HIBERNATING PERIOD

Objective To observe the endocrine cells in the digestive tract of Rana amurensis in and out of hibernation. Methods We used the avidin-biotin-peroxidase complex （ABC） immunohistochemical method and the anti5-HT,anti-SS,anti-PP and anti-SP serums. Results 5-HT,SS and Glu cells were detected in the digestive tract of Rana Amurensis,but GAS , PP and SP cells were not. 5-HT cells distributed widely,observed throughout the digestive tract of Rana Amurensis from esophagus to rectum. The distributive density of 5-HT cells was the highest in pylorus and duodenum in hibernation,but in the pylorus during non-hibernating time.SS cells distributed widely too, observed in the whole digestive tract except the rectum. The highest density of the SS cells was both in the pylorus during the two periods. Glu cells were detected only in gastric cardia, gastric body, jejunum and ileum in hibernation,but also in rectum during the non-hibernating period.The density of Glu distribution peaks in the gastric body.Conclusion The different distribution patterns of endocrine cells in the digestive tract of Rana amurensis in the hibernating and non-hibernating periods may be related to the adaptation to different physiological functions.