Isozymes of the glycolytic and pentose-phosphate pathways in storage tissues of different oilseeds |
| |
Authors: | Robert J Ireland David T Dennis |
| |
Institution: | (1) Department of Biology, Queen's University, K7L 3N6 Kingston, Ontario, Canada;(2) Present address: Department of Biology, Carleton University, K1S 5B6 Ottawa, Ont, Canada |
| |
Abstract: | Isozymes of hexose-phosphate isomerase (HPI; EC 5.3.1.9), pyruvate kinase (PK; EC 2.7.1.40) and 6-phosphogluconate dehydrogenase (6PGDH; EC 1.1.1.44) have been detected in the developing cotyledons of soybean (Glycine max (L.) Merr.), safflower (Carthamnus tinctorius L.) and sunflower (Helianthus annuus L.). In each seed there are two isozymes each of PK and HPI. The isozyme patterns of 6PGDH are more complex: soybean has two forms of the enzyme, safflower three, and sunflower six. In each tissue, at least 25% of the activity of each of the three enzymes is in the plastids. This supports the proposal that the glycolytic and pentose-phosphate pathways are operating in the plastids and that the plastids are the site of long-chain fatty-acid biosynthesis in developing oilseeds.Abbreviations HPI
hexose-phosphate isomerase
- 6PGDH
6-phosphogluconate dehydrogenase
- PK
pyruvate kinase |
| |
Keywords: | Glycolysis Isozymes Oilseeds Pentose-phosphate pathway Plastids Seeds (oil) |
本文献已被 SpringerLink 等数据库收录! |
|