Epitope analysis of peanut allergen Ara h1 with human monoclonal IgM antibody 92-2 |
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Authors: | Hiroshi Shinmoto Yuji Matsuo Yasunori Naganawa Shinichi Tomita Yuko Takano-Ishikawa |
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Institution: | (1) Tamagawa University, Machida Tokyo, 194-8610, Japan;(2) Nagahama Institute of Bio-Science and Technology, Nagahama, 526-0829, Japan;(3) National Food Research Institute, Tsukuba 305-8642, Japan |
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Abstract: | A human-mouse hybridoma clone 92-2 secreting IgM-class human monoclonal antibody to peanut allergen protein Ara h1 was established.
To detect antibody-binding sequences on Ara h1, we synthesized a series of peptides of the Ara h1 protein on a multi-pin apparatus
for the pin-peptide ELISA. The 92-2 human monoclonal antibody was found to recognize a sequence of GREGEQEWGTPGSHVREETS. Further
analysis with shorter pin-peptides with eight amino acid-long showed that the sequence of QEWGTPGS was an essential linear
sequence of this epitope. When the QEW part of the sequence was replaced by alanine, the 92-2 monoclonal antibody did not
bind to the substituted peptide, showing that those amino acids play an important role in the binding of the 92-2 monoclonal
antibody. |
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Keywords: | Human monoclonal antibody Allergen Peanut Epitope IgM |
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