| 水牛腔前卵泡的体外培养方法 |
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| 引用本文： | 潘红平,陈会娟,石德顺,陆凤花,冯贵雪.水牛腔前卵泡的体外培养方法[J].四川动物,2009,28(3). |
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| 作者姓名： | 潘红平 陈会娟 石德顺 陆凤花 冯贵雪 |
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| 作者单位： | 广西大学动物繁殖研究所,南宁,530005 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金，广西自治区科学基金，广西大学博士启动项目 |
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| 摘 要： | 本研究旨在建立一套适合水牛腔前卵泡体外生长发育的培养体系.取用来自本地屠宰场的中国沼泽型水牛卵巢,采用梳刮法回收腔前卵泡,以McCoys 5a作为基础培养液,分别用微孔板培养法、二维培养法、三维培养法进行体外培养.结果表明:不同培养方法对水牛腔前卵泡的体外发育能力有显著差异.培养至10 d,三维培养法的卵泡存活率显著高于微孔板培养法和二维培养法的卵泡存活率(65.05% vs 33.08%,49.52%,P<0.05);二维培养法的卵泡成腔率为1.91%(2/105),三维培养法的卵泡成腔率为1.94%(2/103),而微孔培养法的卵泡未发现成腔;三维培养法的卵泡直径平均增长显著高于微孔板培养法和二维培养法的卵泡直径增长(13.03±5.37 μm vs 7.53±2.26 μm,10.27±4.24 μm,P<0.05).由此可见,三维培养法是水牛腔前卵泡的有效体外培养方法.
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| 关 键 词： | 水牛 腔前卵泡 体外培养 |
Methods for in vitro Culture of Buffalo Preantral Follicles |
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| PAN Hong-ping,CHEN Hui-juan,SHI De-shun,LU Feng-hua,FENG Gui-xue.Methods for in vitro Culture of Buffalo Preantral Follicles[J].Sichuan Journal of Zoology,2009,28(3). |
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| Authors: | PAN Hong-ping CHEN Hui-juan SHI De-shun LU Feng-hua FENG Gui-xue |
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| Institution: | Animal Reproduction Institute;Guangxi University;Nanning 530005;China |
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| Abstract: | A suitable culture system was established for the growth of buffalo preantral follicles in vitro.Buffalo ovaries were obtained from the local slaughterhouse.The comb-scrape method harvested preantral follicles.The base culture medium was McCoy's 5a medium.Preantral follicles were cultured with the micro-plate culture method,two-dimension cultural method and three-dimension cultural method in vitro culture.Effects of culture method on the in vitro development of buffalo preantral follicles were examined.The ... |
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| Keywords: | buffalo preantral follicle in vitro culture |
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