不同亚型一氧化氮合酶在脑缺血/再灌注早期的表达变化

目的:观察脑缺血/再灌注(CI/R)早期缺血区脑组织的内皮型一氧化氮合酶(eNOS)与神经型一氧化氮合酶(nNOS)表达的变化。方法:健康wistar大鼠60只,体重200～280g,由中国医科大学动物中心提供,雌雄各半。随机分为6组(n=10):假手术组、缺血1h组、缺血2h组、再灌注0.5h组、再灌注1h组、再灌注2h组。采用线栓法制作大鼠CI/R模型,免疫组化方法检测缺血区脑组织的eNOS与nNOS蛋白表达情况。结果:与假手术组比较,CI/R模型大鼠脑组织血管内皮细胞内eNOS表达在缺血1h内升高,之后到再灌注2h内持续降低。而nNOS的表达在缺血到再灌注2h内持续上升。结论:CI/R模型中缺血区脑组织的eNOS与nNOS的变化趋势不同,表明一氧化氮在缺血性脑损伤病理过程的作用与一氧化氮合酶亚型的变化有关。

The subgroup change of nitric oxide synthase in earlier period with cerebral ischemia/reperfusion in rats

Objective：To observe the expression of endothelial nitric oxide synthase （eNOS）and nervous nitric oxide synthase （nNOS） in rats during cerebral ischemia/reperfusion （CI/R） and study if change will be happen in subgroup between eNOS and nNOS during earlier period of CI/R.Methods：A total of 60 Wistar rats weighting 200～280 g,supplied by Animal Center of China Medical University,were divided into 6 groups （n=10）（sham operation group;ischemia 1 h、2 h group;reperfusion 0.5 h、1 h、2 h group）.Female and male was half-and-half.Cerebral ischemia/reperfusion injury was induced by a 2-hour suture occlusion of the unilateral middle cerebral artery,immediately after suture withdrawal to allow reperfusion,eNOS and nNOS expressions were examined by the method of immunohistochemistry.Results：eNOS expressions increased in 1-hour during ischemia,keeping up with decreasing until reperfusion 2-hour.While nNOS expressions increased in 2-hour between ischemia and reperfusion.Conclusion：Changes of expression between eNOS and nNOS in rats during cerebral ischemia/reperfusion are different.This may be related with ischemia and reperfusion injury.