羊肚菌液态发酵的研究*

通过摇瓶正交试验研究了圆锥羊肚菌液体发酵的条件，选出最佳培养基配方、接种量、培养温度、pH、通气量，培养时间等参数。在此基础上，应用VIRTIS公司生产的2L－自控发酵罐进行了分批发酵，摸索到了羊肚菌液态深层发酵的基本规律，然后分别以淀粉和玉米粉为碳源进行了补料分批发酵，获得其干细胞产量为25．4g／L和27．4g／L，生长速率为0．45g／（L·h）和0．51g／（L·h），以碳源为基准的产量得率系数（yx／s）为0.72（g／g）和0.75（g／g）等数据。实验结果表明，羊肚菌的液态深层发酵已达到了真菌菌丝液态发酵的正常水平。

A STUDY ON LIQUID FERMENTATION OF MORCHELLA CONICA

Liquid fermentation condition of Morchella conica pers. was studied through orthogonal test using shake flask culture. The optimum parameters of medium component, inoculum size, culture temperature, pH, aeration rate and culture time were given. Liquid fementation was carried out in a 2L-autocontrol fermentor and the essential characteristics were obtained through batch calture. Fed-batch fermentation was carried out by using starch and maize meal as the carbon sources separately. The results were that the yield of dry cell was 25.4g / L, the 27.4g / L, the growth rate was 0.45g / (L·h) and 0.51g / (L·h),and the yield coefficient of dry cell to substrate (yx/s) was 0.72(g / g) and 0.75(g / g), respectively. All these data have come up to the normal level of liquid fermentation of fungal mycelia.