| 糙皮侧耳（平菇）疏水蛋白的分离纯化及其界面自组装特性研究 |
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| 引用本文： | 马爱民,单麟军,杜昭平,谢笔钧.糙皮侧耳（平菇）疏水蛋白的分离纯化及其界面自组装特性研究[J].菌物学报,2007,26(4):557-564. |
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| 作者姓名： | 马爱民 单麟军 杜昭平 谢笔钧 |
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| 作者单位： | 华中农业大学食品科技学院,武汉,430070 |
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| 基金项目： | 国家自然科学基金资助项目(No.39770530,No.30170658) |
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| 摘 要： | 从糙皮侧耳Pleurotus ostreatus Pm039菌丝体中分离纯化到一种疏水蛋白并命名为Po.HYD1,SDS-PAGE显示其分子量约15kDa。Po.HYD1具有高度的表面活性,100μg/mL浓度下能够降低水表面张力至25.5mN/m。在1~100μg/mL浓度范围内存在6μg/mL和24μg/mL两个关键浓度,说明了不同浓度范围内自组装条件的改变。水接触角测定证明了Po.HYD1自组装膜的包被能够逆转固体表面的可湿润性。原子力显微镜分析揭示了Po.HYD1在云母表面形成厚度4.2±0.1nm"小杆层";在高定向热裂解石墨表面形成厚度3.2~3.8nm吸附层;在剧烈振荡诱导下的水溶液中形成形状相似、取向一致但体积大小不等的"耳型"颗粒。
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| 关 键 词： | 糙皮侧耳 疏水蛋白 平衡表面张力 水接触角 原子力显微镜 |
| 文章编号： | 1672-6472(2007)04-0557-0564 |
| 收稿时间： | 2007/5/30 0:00:00 |
| 修稿时间： | 2007年5月30日 |
Purification of hydrophobin from Pleurotus ostreatus and characterization of the interfacial self-assembly property |
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| Authors: | MA Ai-Min SHAN Lin-Jun DU Zhao-Ping and XIE Bi-Jun |
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| Institution: | College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: | A hydrophobin designated as Po.HYD1 was purified from Pleurotus ostreatus Pm039. SDS-PAGE analysis showed an approximate molecular mass of 15kDa. Po.HYD1 showed highly surface activity, and it was able to decrease water surface tension to 25.5mN/m at concentration of 100μg/mL. Two crucial concentrations, 6μg/mL and 24μg/mL, were found by protracting the concentration-surface tension curve in range of 1~100μg/mL, which maybe indicate different conditions concerning interfacial self-assembly. Measurement of water contact angles suggested Po.HYD1 can reverse surface wettability through coating solid materials. Atomic force microscopy revealed Po.HYD1 formed distinct assemblages at different hydrophilic-hydrophobic interfaces. It formed "rodlet layer" with thickness of 4.2±0.1nm on mica surface, formed adsorption layer with thickness of 4.2±0.1nm on highly oriented pyrolytic graphite surface, and formed ear-like particles with similar shape, identical orientation and various sizes in aqueous solution by induction of vigorous shaking. |
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| Keywords: | Pleurotus ostreatus hydrophobin equilibrium surface tension water contact angle atomic force microscopy |
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