| 用ISSR分子标记鉴别东北地区黑木耳生产菌株的研究 |
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| 引用本文： | 张介驰,马庆芳,张丕奇,戴肖东,韩增华,孔祥辉.用ISSR分子标记鉴别东北地区黑木耳生产菌株的研究[J].菌物学报,2007,26(4):534-538. |
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| 作者姓名： | 张介驰 马庆芳 张丕奇 戴肖东 韩增华 孔祥辉 |
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| 作者单位： | 黑龙江省科学院应用微生物研究所,哈尔滨,150010 |
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| 基金项目： | 中国食用菌协会招标研究项目 |
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| 摘 要： | 利用ISSR分子标记对东北地区黑木耳生产菌株进行了分子鉴别,结果表明在选用的20个UBC-ISSR引物中,有10个引物能对供试的27个黑木耳菌株基因组DNA进行扩增,获得的指纹图谱清晰稳定、多态性强。用NTSYS软件进行聚类分析,相似水平在0.75时,可将27个供试黑木耳菌株分为3个组群。研究结果说明ISSR分子标记,可以有效地用于黑木耳生产菌株快速准确鉴别,是黑木耳指纹图谱分析的理想手段。
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| 关 键 词： | ISSR分子标记 黑木耳生产菌株 聚类分析 指纹图谱分析 |
| 文章编号： | 1672-6472(2007)04-0534-0538 |
| 收稿时间： | 2007/3/16 0:00:00 |
| 修稿时间： | 2007年3月16日 |
Identification of cultivated strains of Auricularia auricula from northeastern China by ISSR marker |
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| Authors: | ZHANG Jie-Chi MA Qing-Fang ZHANG Pi-Qi DAI Xiao-Dong HAN Zeng-Hua and KONG Xiang-Hui |
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| Institution: | Institute of Applied Microbiology Heilongjiang Academy of Sciences, Harbin 150010, China;Institute of Applied Microbiology Heilongjiang Academy of Sciences, Harbin 150010, China;Institute of Applied Microbiology Heilongjiang Academy of Sciences, Harbin 150010, China;Institute of Applied Microbiology Heilongjiang Academy of Sciences, Harbin 150010, China;Institute of Applied Microbiology Heilongjiang Academy of Sciences, Harbin 150010, China;Institute of Applied Microbiology Heilongjiang Academy of Sciences, Harbin 150010, China |
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| Abstract: | Inter simple sequence repeat (ISSR) method was used to distinguish the strains of Auricularia auricula in northeastern China. The result showed that 10 primers selected from 20 ISSR primers tested could produce distinct and polymorphic fingerprints of 27 cultivated strains of A. auricula. The result from the clustering analysis by NTSYS showed that these cultivated strains could be divided into three groups at similar level of 0.75. The result indicated that ISSR marker was an effective tool for strain identification and fingerprint analysis of A.auricula. |
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| Keywords: | ISSR cultivated strains of Auricularia auricula molecular identification clustering analysis fingerprint analysis |
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