利用RAPD技术对木耳属菌株进行分类鉴定的研究

利用ＲＡＰＤ技术对木耳属不同种和种内不同菌株进行了分子鉴定。结果表明，在供试的２６个随机引物中，有１０个引物可扩增出清晰、稳定的ＤＮＡ带型，其中引物Ｓ４和Ｓ６与供试木耳属菌株的基因组ＤＮＡ结合位点少而保守，扩增图谱具有种的特异性，可用于种的快速准确鉴定；其综随机引物的扩增ＤＮＡ多态性较强，可用于种内不同菌株的鉴定；聚类分析表明，在７５％相似水平下，可将供试的木耳属菌株分成四大类，其中有三大类各代表一个形态鉴定的种。研究结果表明了ＲＡＰＤ技术可有效地用于木耳属种或菌株的快速准确鉴定。

CLASSIFICATION AND IDENTIFICATION OFSPECIES AND STRAINS IN AURICULARIABY USING RAPD

RAPD technique was used to characterize and classify the species and sabins of the genusAuricularia. The results showed that the annealing sites between random primer S4 or S6 and genomic DNA ofdifferent species were rare and conserved. The amplification patterns were species-specific. The two primers couldbe used in species identification directly; other tested primers could generate more polymorphic DNA and couldbe used in strain identification. Cluster analysis results showed that at 75% similarity level, all strains could begrouped into four clusters. Of the four clusters, three represented one morphological species respectively. RAPDmay provide a useful and rapid tool for identifying species and stains of Auricularia.