芳香杯伞生物学特性及驯化栽培

以采自于河北省平泉市的一株野生食用菌Clitocybe fragrans的菌丝体为材料,对其营养及环境特性进行研究.检测了不同碳源、氮源、C/N、无机盐在固体培养条件下对芳香杯伞菌丝生长的影响,单因素及正交试验结果表明,实验范围内,芳香杯伞最适碳源为蔗糖、最适氮源为酵母浸粉、最适C/N为40∶1、最适无机盐为硫酸镁.正...

Biological characteristics and cultivation of Clitocybe fragrans

The nutritional and environmental characteristics of a wild Clitocybe fragrans obtained from Pingquan City in Hebei Province were studied. The effects of carbon source, nitrogen source, C/N, and inorganic salt on the mycelial growth were investigated by single factor test and optimized by orthogonal experiments. The results revealed that the optimum carbon source, nitrogen source, C/N and inorganic salt were sucrose, yeast extract, 40:1, and magnesium sulphate respectively. The culture medium formula was as follows: sucrose 17.3 g, yeast extract 1.8 g, potassium dihydrogen phosphate 3 g, anhydrous magnesium sulfate 1.5 g, agar 20 g, and water 1 L. The mycelia grew well at pH 5.0-5.5 and incubation temperature of 19-25 °C under dark condition. The domestic cultivation of the mushroom was executed successfully. The suitable stock culture medium for propagated cultivation was composed of 100 g of compost substrate (boiled juice), 200 g of potato (boiled juice), 20 g of glucose, 3 g of potassium dihydrogen phosphate, 3 g of peptone, 1.5 g of anhydrous magnesium sulfate, 20 g of agar and 1 L of water. The mother spawn medium was composed of sorghum 98%, glucose 1%, and gypsum 1%. The spawn substrate was composed of 98% compost substrate, 1% gypsum and 1% glucose with 55% moisture capacity. Using method of sack cultivation, the mycelia were sackful in 35-40 days after inoculation. The primordia were formed 8-10 days later, and grew into fruit bodies after 7-8 days.