柱状田头菇EST-SSR的开发及应用研究

借鉴香菇EST序列设计的40对引物对柱状田头菇进行了EST-SSR引物通用性研究。结果显示，挑选的28对引物中多态性好且稳定的有13对，通用率达46.4%。PCR扩增产物获得具有多态性条带81条，每对引物扩增条带的数目范围为2–14，平均5.86条，扩增片段大小在100–400bp之间。多态信息含量（PIC）在0.3750–0.8032，平均0.7084。研究结果表明，EST-SSR分子标记在食用菌的遗传多样性和比较基因组学研究中起到重要的作用。

EST-SSR markers in Agrocybe cylindracea and its application

For the development of EST-SSR markers in Agrocybe cylindracea and the application of the marker in characterization of different mushrooms, 40 pairs of primers were designed based on the expressed sequence tags obtained from the shiitake Lentinula edodes. The results showed that 13 of 28 selected primer pairs could reveal high and stable polymorphisms among Agrocybe cylindracea varieties, which accounted for 46.4% of primers selected. Amplified PCR product has a total of 81 polymorphic bands, and the number of bands’ range is 2–14 with an average of 5.86 bands. Amplified fragment length is between 100–400bp. The polymorphism information content (PIC) varied from 0.3750 to 0.8032, with an average of 0.7084. The results of this study indicated that EST-SSR markers could be used in the genetic diversity and comparative genomics studies among different edible fungus species.