Comparison of intracellular glutathione and related antioxidant enzymes: Impact of two glycosylated whey hydrolysates |
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| Institution: | 1. Key Laboratory of Dairy Science (Northeast Agricultural University), Ministry of Education, Harbin 150030, PR China;2. College of Resources and Environmental Science, Northeast Agriculture University, Harbin, 150030, PR China;1. Unit of Genetics, Department of Biotechnology – Plant Biology, Universidad Politecnica de Madrid, Madrid, 28040, Spain;2. Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991, Moscow, Russia;3. QAAFI, University of Queensland, Brisbane, QLD 4072, Australia;1. Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, Haryana, India;2. Department of Biochemistry, M.D. University, Rohtak, Haryana, India;1. State Key Laboratory of Bioreactor Engineering, Department of Food Science and Technology, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, People’s Republic of China;2. State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, People’s Republic of China;3. Shanghai Collaborative Innovation Center for Biomanufacturing (SCICB), Shanghai 200237, People’s Republic of China;1. Department of Chemical and Biochemical Engineering, Technical University of Denmark, Soltofts Plads, Building 227, 2800 Kgs. Lyngby, Denmark;2. Novozymes A/S, Vegetable oil processing R&D, Krogshojvej 36, 2800 Lyngby, Denmark;1. Agrarian and Environmental Sciences Center, Federal University of Maranhão, Chapadinha, Maranhão, Brazil;2. Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil;3. Postgraduate Program in Microbial Biology, CEUMA, University of São Luis, Maranhão, Brazil;4. Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará, Brazil;5. Educator Training Institute, Federal University of Cariri, Brejo Santo, Ceará, Brazil;1. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, PR China;2. Research Center of Environmental Pollution Control Engineering Technology, Chinese Research Academy of Environmental Sciences, Beijing, 100012, PR China;3. College of Water Sciences, Beijing Normal University, Beijing, 100875, PR China;4. School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijing, 100083, PR China |
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| Abstract: | The effects of two glycosylated whey hydrolysates (GWH-Gal A and GWH-Gal B) on glutathione (GSH) and related antioxidant enzymes in SGC-7901 cells were evaluated. Two whey glycosylated hydrolysates promoted an increase in reduced glutathione (GSH) in normal SGC-7901 cells. GSH, glutathione peroxidase (GPx), γ-glutamine cysteine synthetaase (γ-GCS), and catalase (CAT) at 1.0 and 2.0 mg/mL in normal SGC-7901 cells were higher in the GWH-Gal A group than in the GWH-Gal B group (P < 0.05). Compared with GWH-Gal B, GWH-Gal A more strongly inhibited decreases in intracellular GSH, GPx, γ-GCS, CAT, and superoxide dismutase (SOD) in H2O2-induced SGC-7901 cells. Compared with GWH-Gal B, GWH-Gal A at 1.0 and 2.0 mg/mL effectively inhibited increases in lactate dehydrogenase (LDH) and malondialdehyde (MDA) in H2O2-induced SGC-7901 cells (P < 0.05). Therefore, GSH content and related antioxidant enzyme activity levels (GPx, γ-GCS, CAT, SOD) in both normal and H2O2-induced SGC-7901 cells were considerably stronger in the GWH-Gal A group than in the GWH-Gal B group. |
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| Keywords: | Glycosylated whey hydrolysates Glutathione Antioxidant enzymes SGC-7901 cells |
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