用AFLP标记构建白桦遗传连锁图谱

用AFLP的方法分析中国白桦×欧洲白桦的78个F1个体，并按照拟测交作图策略，建立了中国白桦和欧洲白桦遗传连锁图谱。从群体的45对引物组合中分离出343个分离位点，χ^2检验表明，其中有311个符合1：1拟测交分离位点。在这些位点中168个来自中国白桦，143个来自欧洲白桦。软件分析表日月，中国白桦的168个位点构成9个连锁群，11个三联体和14个连锁对，55个为非连锁位点，连锁标记覆盖的总距离为1909．2cM，平均图距为16．9cM；来自欧洲白桦的143个位点构成12个连锁群，4个三联体和9个连锁对，21个为非连锁位点，连锁标记覆盖的总距离为1857．3cM，平均图距为15．2cM。

Construction of Genetic Linkage Maps of Silver Birch Based on AFLP Markers

Based on the inheritance and segregation of amplified fragment length polymorphism （AFLP） markers, the first middensity linkage map for silver birch was constructed by using a pseudotestcross mapping strategy. A segregating population including 78 progenies from the cross between Betula platyphylla and B. pendula was obtained. 45 primer pair combinations were selected to generate AFLP markers within a sample of 78 F1 progenies and 343 segregating sites were identified. Among the identified sites, 311 segregating sites belonged to 1：1 segregating site including 143 sites for B. pendula and 168 sites for B. platyphylla. The resulting linkage maps were consisted of 168 marker sites in 9 groups, 11 triples, 14 pairs and 55 mon-linkage sites for B. platyphylla, which covered the map distance about 1 909.2 cM （Kosambi units）. The average map distance between adjacent markers was 16.9 cM. 143 linked marker-sites of B. pendula were mapped onto 12 groups, 4 triples, 9 pairs and 21 mon-linkage sites, which covered the map distance about 1 857.3 cM. The average map distance between adjacent markers was 15.2 cM.