| 30个枣树种质资源遗传多样性的ISSR分析 |
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| 引用本文： | 侯思宇,孙朝霞,申洁,王玉国,韩渊怀.30个枣树种质资源遗传多样性的ISSR分析[J].植物生理学通讯,2011(3):275-280. |
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| 作者姓名： | 侯思宇 孙朝霞 申洁 王玉国 韩渊怀 |
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| 作者单位： | 山西农业大学农学院生物技术系;山西农业大学生物工程研究所; |
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| 基金项目： | 山西省留学人员科研资助项目(2010041); 山西农业大学引进人才基金(416568) |
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| 摘 要： | 取30个枣树品种进行ISSR分子标记分析，其扩增条带分别进行琼脂糖和聚丙烯酰胺凝胶电泳检测，以期获得不同产地品种之间的遗传多态性。从100条选择扩增的ISSR引物中筛选出17条扩增清晰、重复性和稳定性好的引物，选取其中8条扩增条带多态性强的引物进行遗传聚类分析。结果表明：（1）1％琼脂糖凝胶电泳和5％聚丙烯酰胺凝胶电泳检测扩增总条带数分别为72和127条，其中多态性条带分别为51条和113条，多态性条带比率（PPB）分别为70．8％和88．9％。（2）基于UPGMA软件对30个品种的遗传差异性分析表明，8个ISSR引物可以将枣树品种之间遗传差异明显区分开来。两种电泳检测方法相比较，聚丙烯酰胺凝胶电泳检测可获得较为精细的枣树品种间遗传图谱。其遗传相似系数范围在0．56～1．00之间，以0．62为最低遗传相似系数，可将30个枣树品种分成3个大类，6个亚类，为进一步研究枣树品种间分类、起源进化关系和分子辅助育种奠定基础。
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| 关 键 词： | 枣 ISSR 遗传多样性 |
Genetic Variation among Thirty Ziziphus jujuba Mill.Cultivars as Revealed by ISSR Marker Assays |
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| HOU Si-Yu,SUN Zhao-Xia,SHEN Jie,WANG Yu-Guo,HAN Yuan-Huai,.Genetic Variation among Thirty Ziziphus jujuba Mill.Cultivars as Revealed by ISSR Marker Assays[J].Plant Physiology Communications,2011(3):275-280. |
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| Authors: | HOU Si-Yu SUN Zhao-Xia SHEN Jie WANG Yu-Guo HAN Yuan-Huai |
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| Institution: | HOU Si-Yu1,SUN Zhao-Xia1,SHEN Jie1,WANG Yu-Guo1,HAN Yuan-Huai1,2,1Department of Biotechnology,School of Agriculture,2Bioengineering Institute,Shanxi Agricultural University,Taigu,Shanxi 030801,China |
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| Abstract: | Genetic diversity among 30 accessions of jujube(Ziziphus jujuba) were investigated with inter simple sequence repeats(ISSR) technique,using agarose gel electrophoresis and polyacrylamide gel electrophoresis(PAGE).ISSR analysis was carried out 8 random primers generated from 17 polymorphic primers.The results showed that:(1) 72 and 127 DNA bands were amplified and detected by 1% agarose gel electrophoresis and 5% PAGE respectively,with the percentage of polymorphic bands(PPB)(88.9%) in PAGE detection higher ... |
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| Keywords: | jujube ISSR genetic diversity |
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