| 机械刺激诱导的烟草悬浮细胞一氧化氮产生途径及其与C矿和钙调素的关系 |
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| 引用本文： | 柯学,李忠光,刘娴,龚明.机械刺激诱导的烟草悬浮细胞一氧化氮产生途径及其与C矿和钙调素的关系[J].植物生理学通讯,2011(1):85-90. |
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| 作者姓名： | 柯学 李忠光 刘娴 龚明 |
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| 作者单位： | 中国农业大学水利与土木工程学院;云南师范大学生命科学学院;云南师范大学生物能源持续开发利用教育部工程研究中心;云南师范大学云南省生物质能与环境生物技术重点实验室; |
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| 基金项目： | 国家自然科学基金(30460016); 云南省教育厅基金(07Z11860,09Y0145) |
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| 摘 要： | 通过提高摇床转速对烟草细胞施加机械刺激（Ms）可诱导其胞内一氧化氮（No）的快速产生和一氧化氮合酶（Nos）活性的提高，这种MS诱导的NO产生可被N0清除剂cPTIO和NOS抑制剂L-NMMA显著抑制。此外，Ca2＋螯合剂EGTA、质膜Ca＋通道阻断剂La3＋、胞内Ca2＋通道拮抗剂钌红，以及钙调素抑制剂CPZ和TFP预处理均不同程度地抑制了机械刺激诱导的烟草细胞NO的产生，而机械刺激过程中钙调素活性显著上升并与NOS活性和NO含量的变化相一致。这些结果暗示着（类）Nos酶催化的精氨酸依赖途径可能是机械刺激诱发烟草细胞NO产生的主要途径，Ca2＋／CAM可能通过调节（类）NOS活性来调控No的产生。
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| 关 键 词： | 机械刺激 一氧化氮 一氧化氮合酶 钙 钙调素 烟草细胞 |
Pathways of Mechanical Stimulation-Induced Nitric Oxide Production and Their Relationship with Ca~(2+) and Calmodulin in Tobacco (Nicotiana tobacum L.) Suspension Culture Cells |
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| KE Xue,LI Zhong-Guang,,LIU Xian,GONG Ming,College of Water Conservancy & Civil Engineering,China Agricultural University,Beijing ,China,School of Life Sciences,Engineering Research Center of Sustainable Development , Utilization of Biomass Energy,Ministry of Education,Key Laboratory of Biomass Energy , Environmental Biotechnology,Yunnan Province,Yunnan Normal University,Kunming.Pathways of Mechanical Stimulation-Induced Nitric Oxide Production and Their Relationship with Ca~(2+) and Calmodulin in Tobacco (Nicotiana tobacum L.) Suspension Culture Cells[J].Plant Physiology Communications,2011(1):85-90. |
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| Authors: | KE Xue LI Zhong-Guang LIU Xian GONG Ming College of Water Conservancy & Civil Engineering China Agricultural University Beijing China School of Life Sciences Engineering Research Center of Sustainable Development Utilization of Biomass Energy Ministry of Education Key Laboratory of Biomass Energy Environmental Biotechnology Yunnan Province Yunnan Normal University Kunming |
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| Institution: | KE Xue1,2,LI Zhong-Guang2,3,4,LIU Xian2,GONG Ming2,1College of Water Conservancy & Civil Engineering,China Agricultural University,Beijing 100083,China,2School of Life Sciences,3Engineering Research Center of Sustainable Development and Utilization of Biomass Energy,Ministry of Education,4Key Laboratory of Biomass Energy and Environmental Biotechnology,Yunnan Province,Yunnan Normal University,Kunming 650092 |
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| Abstract: | Application of mechanical stimulation (MS) on tobacco (Nicotiana tobacum) suspension culture cells led to rapid production of intracellular nitric oxide (NO) and increased activity of nitric oxide synthase (NOS),but this MS-induced NO production could be significantly inhibited by the NO scavenger cPTIO and NOS inhibitor L-NMMA.In addition,the Ca2+ specific chelator EGTA,plasma membrane Ca2+ channel blocker La3+,intracellular Ca2+ channel antagonist ruthenium red,and the calmodulin inhibitors chlorpromazine... |
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| Keywords: | mechanical stimulation nitric oxide nitric oxide synthase calcium calmodulin tobacco suspension cells |
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