| 肥荚红豆种子无菌诱导植株再生及快速繁殖 |
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| 引用本文： | 彭绿春,王丽花,马双喜,苏艳,张艺萍,宋杰,瞿素萍.肥荚红豆种子无菌诱导植株再生及快速繁殖[J].植物生理学通讯,2013(9):917-922. |
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| 作者姓名： | 彭绿春 王丽花 马双喜 苏艳 张艺萍 宋杰 瞿素萍 |
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| 作者单位： | [1]云南省农业科学院花卉研究所,昆明650205 [2]农业部花卉产品质量监督检验测试中心昆明,650205 [3]云南省花卉育种重点实验室,昆明650205 [4]云南花卉技术工程研究中心,昆明650205 [5]云南省玉溪市易门县林业局,云南玉溪651100 |
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| 基金项目： | 资助云南省科技计划项目（2011EB111）. |
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| 摘 要： | 以肥荚红豆种子为外植体，研究了种子预处理、诱导萌发、不定芽增殖、壮苗、生根的方法，摸索培养基中激素的最佳配比，建立了肥荚红豆组织培养快速繁殖技术。结果表明：5％～10％NaOH溶液浸泡种子1h的预处理有利于促进肥荚红豆种皮的剥离和种子萌发；适宜于种子萌发的培养基为MS（Read）＋6-BA0．5mg·L^-1＋NAA0．1mg·L^-1，萌发率可达100％；最佳增殖培养基为Read＋6．BA1．0～1．5mg·L^-1＋KT0．1mg·L^-1＋NAA0．1mg·L^-1，增殖数可达每瓶23个以上；最佳壮苗培养基为Read＋6．BA0-0．5mg·L^-1＋NAA0．1mg·L^-1：最佳生根培养基为Read＋IAA1．0mg·L^-1，其生根率可达90．54％。研究结果提高了肥荚红豆的繁殖效率，有效促进肥荚红豆组培规模化育苗技术的成熟与推广应用，对推动其产业开发应用具有重要意义。
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| 关 键 词： | 肥荚红豆 种子 植株再生 高效繁殖 |
Plant Regeneration through Seed Asptic Germination and Rapid Propagation Technology of Ormosia fordiana Oliv. |
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| PENG Lu-Chun,WANG Li-Hua,MA Shuang-Xi,SU Yan,ZHANG Yi-Ping,SONG Jie,QU Su-Ping.Plant Regeneration through Seed Asptic Germination and Rapid Propagation Technology of Ormosia fordiana Oliv.[J].Plant Physiology Communications,2013(9):917-922. |
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| Authors: | PENG Lu-Chun WANG Li-Hua MA Shuang-Xi SU Yan ZHANG Yi-Ping SONG Jie QU Su-Ping |
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| Institution: | 1Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, China; 2Supervision and Testing Centre for Flowers of Ministry of Agriculture (Kunming), Kunming 650205, China; 3Yunnan Flower Breeding Key Laboratory, Kunming 650205, China; 4yunnan Flower Research and Development Center, Kunming 650205, China; 5Forestry Bureau of lqmen County, Yuxi, Yunnan 651100, China |
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| Abstract: | The plant regeneration system of Ormosiafordiana Oliv. was studied with the pretreatment and germination induction of seed, multiplication and hardening of adventitious bud, rooting of the shoot. The results showed that the best seed germination medium was MS （Read）＋6-BA 0.5 mg.L^-1＋NAA 0.1 mg^-L] with 100% of germination rate, and the best multiplication medium was Read＋6-BA 1.0-1.5 mg.L^-1KT 0.1 mg.L^-1＋NAA 0.1 mg.L^-1 which has more than twenty-three multiplication buds in each bottle. Read＋6-BA 0-0.5 mg.L^-1＋ NAA 0.1 mg.L^-1 was the best hardening medium. The best rooting medium was Read＋IAA 1.0 mg.Ll with 90.54% of the rooting rate. The research would promote the reproductive efficiency of Ormosiafordiana Oliv.. It improved the maturity and application of large-scale seedling technology, and had the vital significance on development and application ofOrmosiafordiana Oliv. |
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| Keywords: | Ormosiafordiana Oliv seed plant regeneration effective multiplication |
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