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玻璃化法超低温保存蛇莓茎尖
引用本文:薄涛,王子成,柴星星.玻璃化法超低温保存蛇莓茎尖[J].植物生理学通讯,2010(9):953-956.
作者姓名:薄涛  王子成  柴星星
作者单位:河南大学生命科学学院,河南开封475004
基金项目:国家自然科学基金(30900973)
摘    要:本文采用玻璃化法对蛇莓离体茎尖超低温保存进行了初步探讨。研究了低温锻炼时间、预培养时间、预处理时间、玻璃化液处理时间和液氮保存时间对超低温保存后成活率的影响。经优化,蛇莓的最高成活率可达(42.00±2.74)%。

关 键 词:蛇莓  茎尖  超低温保存  玻璃化  成活率

Cryopreservation of Duchesnea indica (Andr.) Focke Shoot-Tips by Vitrification
BO Tao,WANG Zi-Cheng,CHAI Xing-Xing.Cryopreservation of Duchesnea indica (Andr.) Focke Shoot-Tips by Vitrification[J].Plant Physiology Communications,2010(9):953-956.
Authors:BO Tao  WANG Zi-Cheng  CHAI Xing-Xing
Institution:(College of Life Science,Henan University,Kaifeng,Henan 475004,China )
Abstract:A procedure had been studied on cryopreservation of Duchesnea indica (Andr.) Focke shoot-tips in vitro by vitrification. The influence of the treatment periods of cold temperature acclimation,pre-culture,pretreatment,PVS2 and cryopreservation in liquid nitrogen on survival rate after cryopreservation was studied. The best survival rate reached to (42.00±2.74)%.
Keywords:Duchesnea indica  shoot-tip  cryopreservation  vitrification  survival rate
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