| N-氨甲酰基-D-氨基酸酰胺水解酶的固定化工艺 |
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| 引用本文： | 张小飞,朱银惠,王璐璐.N-氨甲酰基-D-氨基酸酰胺水解酶的固定化工艺[J].生物加工过程,2009,7(4):46-49. |
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| 作者姓名： | 张小飞 朱银惠 王璐璐 |
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| 作者单位： | 1. 河北工业职业技术学院,石家庄,050091
2. 山东师范大学,外国语学院,济南,250014 |
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| 摘 要： | 以TJS环氧基树脂作为载体对N-氨甲酰基-D-氨基酸酰胺水解酶进行固定化,最佳工艺条件为：1g树脂载体大约对应133U酶液,蛋白质量浓度0.35mg/mL,固定时间15h,温度28℃,pH7.5,固定化酶活达到58.5U。蛋白固定率可达97.4%,酶活回收率达到49.3%,得到的固定化酶使用半衰期达到26批。
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| 关 键 词： | D-对羟基苯甘氨酸 N-氨甲酰基-D-氨基酸酰胺水解酶 固定化 |
Immobilization of N-carbamyl-D-amino acid amidohydrolase |
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| ZHANG Xiao-fei,ZHU Yin-hui,WANG Lu-lu.Immobilization of N-carbamyl-D-amino acid amidohydrolase[J].Chinese Journal of Bioprocess Engineering,2009,7(4):46-49. |
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| Authors: | ZHANG Xiao-fei ZHU Yin-hui WANG Lu-lu |
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| Institution: | 1. Hebei College of Industry and Technology, Shijiazhuang 050091, China; 2. College of Foreign Languages, Shandong Normal University, Jinan 250014, China) |
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| Abstract: | Epoxy resin TJS was used as carriers for immobilization of N-carbamyl-D-amino acid amidohydrolase(DCase).The optimized conditions were as follows:133 U/g TJS resin, protein eoncentration 0.35 ms/mL, reaction time 15 h,temperature 28℃,and pH 7.5.Under the optimum conditions, the activity of the immobilized enzyme was 58.5 U/g,the immobilization yield was 97.4%,and the enzyme activity recovery was 49.3%.The immobilized DCase was used for 26 times. |
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| Keywords: | D-hydroxyphenylglycine N-carbamyl-D-amino acid amidohydrolase immobilization |
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