| 十二烷基硫酸钠协同微波-酶法提取布渣叶总黄酮 |
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| 引用本文： | 马婉婉,骆开荣,班颖,刘曦,韩伟.十二烷基硫酸钠协同微波-酶法提取布渣叶总黄酮[J].生物加工过程,2011,9(6):35-38. |
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| 作者姓名： | 马婉婉 骆开荣 班颖 刘曦 韩伟 |
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| 作者单位： | 华东理工大学中药现代化工程中心,上海,200237 |
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| 基金项目： | 国家自然科学基金资助项目，国家大学生创新计划资助项目 |
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| 摘 要： | 采用十二烷基硫酸钠(SDS)协同微波-酶法提取布渣叶总黄酮,选取微波辐射时间(X1)、SDS质量分数(X2)、液固比(X3)、恒温水浴提取时间(X4)、酶解时间(X5)进行U15(54×3)混合水平均匀试验设计,利用偏最小二乘法(PLS)处理试验数据,建立数学模型,确定提取的最优条件并进行验证；另外,将优化后的提取结果与其他提取方法进行比较.研究结果表明:优化的提取条件为X1=1min、X2=0.16％、X3=30.75 mL/g、X4=50 min、X5=30 min.与其他提取方法相比,该法提取效率高,环保无污染,具有一定的应用价值.
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| 关 键 词： | 十二烷基硫酸钠(SDS) 酶法 布渣叶 微波 总黄酮 |
Extraction of total flavones from Microcos paniculata Linn.by combining sodium dodecyl sulfate with microwave and enzyme |
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| MA Wanwan,LUO Kairong,BAN Ying,LIU Xi,HAN Wei.Extraction of total flavones from Microcos paniculata Linn.by combining sodium dodecyl sulfate with microwave and enzyme[J].Chinese Journal of Bioprocess Engineering,2011,9(6):35-38. |
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| Authors: | MA Wanwan LUO Kairong BAN Ying LIU Xi HAN Wei |
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| Institution: | MA Wanwan,LUO Kairong,BAN Ying,LIU Xi,HAN Wei(Engineering Center for Tradition Chinese Medicine Moderniziation,East China University ofScience and Technology,Shanghai 200237,China) |
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| Abstract: | In order to obtain the best method for extraction of total flavones,the uniform design method U15（54×3） was used to investigate the effect of microwave extraction time（X1）,sodium dodecyl sulfate concentration（X2）,liquid to solid ratio（X3）,enzymatic time（X4）,and water extraction time（X5） on the yield of total flavones from Microcos paniculata Linn.by combining surfactant with the microwave and the enzyme,a mathematical model was established and the optimal process conditions were obtained with PLS procedure.The optimal extraction parameters of total flavones were as follows： X1=1 min,X2=0.16%,X3=30.75 mL/g,X4=50 min,X5=30 min.The extraction process had advantages of high-efficiency and safety. |
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| Keywords: | sodium dodecyl sulfate enzyme Microcos paniculata Linn microwave total flavones |
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