| 双波长法用于磺酸型树脂吸附BSA和trypsin的研究 |
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| 引用本文： | 魏荣卿,邵勇军,刘晓宁,张婷婷,姚忠.双波长法用于磺酸型树脂吸附BSA和trypsin的研究[J].生物加工过程,2004,2(3):56-59. |
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| 作者姓名： | 魏荣卿 邵勇军 刘晓宁 张婷婷 姚忠 |
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| 作者单位： | 南京工业大学,制药与生命科学学院,南京,210009 |
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| 基金项目： | 江苏省科技攻关项目 (BE2 0 0 10 40 ) |
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| 摘 要： | 考察了H^ 和Na^ 变化对紫外吸收单波长法、双波长法和考马斯亮蓝法的影响。结果表明双波长法比考马斯亮蓝法和单波长法稳定性要好。选择双波长法用于磺酸型离子交换树脂吸附胰蛋白酶(trypsin)和牛血清白蛋白(BSA)的检测，结果表明：交换容量为4-88mmol／g的树脂对BSA和trypsin的吸附速率和饱和吸附量均高于交换容量为3．17mmol／g的树脂。
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| 关 键 词： | 双波长法 磺酸型离子交换树脂 牛血清白蛋白 胰蛋白酶 |
| 文章编号： | 1672-3678(2004)03-0056-04 |
| 修稿时间： | 2004年7月4日 |
Study on the dual-wavelength spectrophotometric determination of adsorption of BSA and trypsin on sulfuric acidic cation exchange resins |
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| WEI Rong-qing,SHAO Yong-jun,LIU Xiao-ning,ZHANG Ting-ting,YAO Zhong.Study on the dual-wavelength spectrophotometric determination of adsorption of BSA and trypsin on sulfuric acidic cation exchange resins[J].Chinese Journal of Bioprocess Engineering,2004,2(3):56-59. |
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| Authors: | WEI Rong-qing SHAO Yong-jun LIU Xiao-ning ZHANG Ting-ting YAO Zhong |
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| Abstract: | The influence of H and Na on the mono - wavelength spectrophotometry,dual-wavelength spectrophotometry and Bradford me thod was investigated The results showed that the dual-wavelength method was more stable than other two methods The dual-wavelength method was applied i n the determination of adsorpt io n of BSA and trypsin on sulfuric acidic cation exchange resins,the resin with e xchange capacity of 488 mmol/g has higher maximum adsorptive capacity and adso rptive velocity of BSA and trypsin than the resin of 488 mmol/g |
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| Keywords: | dual-wavelength spectrophotometry sulfuric acidic c ation exchange resin BSA trypsin |
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