| 辅酶Q_(10)产生菌发酵条件的优化 |
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| 引用本文： | 邵玲莉,石玲玲.辅酶Q_(10)产生菌发酵条件的优化[J].生物加工过程,2010,8(2):18-22. |
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| 作者姓名： | 邵玲莉 石玲玲 |
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| 作者单位： | 金华职业技术学院,生物制药技术教研室,金华,321007 |
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| 摘 要： | 对辅酶Q10生产菌株鞘氨醇单胞菌YZ0803的发酵条件进行优化,确定发酵时间为90 h,250 mL摇瓶装液量为30 mL。培养基组成(质量分数,下同):葡萄糖1.5%,淀粉2.5%,黄豆饼粉2.5%,(NH4)2SO40.5%,NaCl0.03%,K2HPO40.02%,MgSO40.005%。优化后的辅酶Q10产量达到192 mg/L,比采用基础培养基的产量(138mg/L)提高了39.13%。
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| 关 键 词： | 辅酶Q10 发酵 优化 正交试验 |
Optimization of fermentation conditions for production of CoQ_(10) |
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| SHAO Ling-li,SHI Ling-ling.Optimization of fermentation conditions for production of CoQ_(10)[J].Chinese Journal of Bioprocess Engineering,2010,8(2):18-22. |
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| Authors: | SHAO Ling-li SHI Ling-ling |
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| Institution: | SHAO Ling-li,SHI Ling-ling(Department of Biopharmaceutical Sciences,JinHua College of Profession , Technology,Jinhua 321007,China) |
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| Abstract: | The culture conditions of the production of CoQ10 by YZ0803 were optimized.The optimal conditions were as follows: fermentation time 90 h,glucose 1.5%,corn starch 2.5%,bean cake 2.5%,(NH4)2SO4 0.5%,NaCl 0.03%,K2HPO4 0.02% and MgSO4 0.005%.The optimum volume of the medium was 30 mL liquid in 250 mL flask.Under the optimized fermentation conditions,the CoQ10 yield reached 192 mg/L,it inereased 39.13% than that under the original conditions. |
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| Keywords: | CoQ_(10) fermentation optimization orthogonal design |
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