| 液体悬浮培养促进铁皮石斛原球茎高效诱导、增殖的研究 |
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| 引用本文： | 苏钛,张晓南.液体悬浮培养促进铁皮石斛原球茎高效诱导、增殖的研究[J].中国野生植物资源,2009,28(4):54-56. |
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| 作者姓名： | 苏钛 张晓南 |
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| 作者单位： | 云南省药物研究所,云南,昆明,650111 |
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| 基金项目： | 云南省昆明市西山区科技计划项目 |
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| 摘 要： | 用正交设计方法对铁皮石斛原球茎具有高效增殖影响的激素（BA，NAA，KT，马铃薯汁）以及配比进行研究，结果表明：以茎段为外植体在培养基Ms＋BA2．0mg／L＋NAA2．0mg／L＋马铃薯汁10％＋糖3％，具有很好的原球茎诱导作用，50d后诱导率达95．20％；原球茎在1／2MS＋BA2．0mg／L＋NAA1．0mg／L＋KT0．5mg／L＋糖3％的培养基上，以液体悬浮培养，原球茎增殖达49．032g／50d。
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| 关 键 词： | 铁皮石斛 原球茎 悬浮培养 |
Study on the Induction and Proliferation of Protocorm in Dendrobium officinale by Suspension Culture |
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| Su Tai,Zhang Xiaonan.Study on the Induction and Proliferation of Protocorm in Dendrobium officinale by Suspension Culture[J].Chinese Wild Plant Resources,2009,28(4):54-56. |
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| Authors: | Su Tai Zhang Xiaonan |
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| Institution: | (Yunnan Institute of Materia Medica, Kunming 650111 China) |
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| Abstract: | To study on the effects of plant growth regulator （ BA, NAA, KT, and Potato juice） on the induction and proliferation of Dendrobium officinale protocorm by orthogonal design. The results showed that the optimum induction medium is MS ＋ BA2.0 mg/L ＋ NAA2.0 mg/L ＋ potato juice10% ＋ sugar 3%, the induction rate of protocorm is 95.20% after 50 days; the plants are transplanted in the proliferation medium of 1/2MS ＋ BA2.0 mg/L ＋ NAA1.0 mg/L ＋ KT0.5 mg/L ＋ sugar 3% after the protocorm are inducted. Proliferation rate of Dendrobium officinale protocorm in liquid medium is 49. 032 g/50d. |
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| Keywords: | Dendrobium offinale protocorm suspension culture |
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