|
|||||
|
|
| NGAL蛋白诱导食管癌细胞发生自噬 | |
| 引用本文： | 张丕显,方王楷,许丽艳,蒋纪恺,沈忠英,杜则澎,陆晓峰,周飞,谢剑君,吴炳礼,崔有宏,谢东,李恩民.NGAL蛋白诱导食管癌细胞发生自噬[J].生物化学与生物物理进展,2009,36(8):978-986. |
| 作者姓名： | 张丕显 方王楷 许丽艳 蒋纪恺 沈忠英 杜则澎 陆晓峰 周飞 谢剑君 吴炳礼 崔有宏 谢东 李恩民 |
| 作者单位： | 1. 汕头大学医学院生物化学与分子生物学教研室,汕头,515041 2. 汕头人学医学院肿瘤病理研究室,汕头,515041 3. 汕头大学医学院病理生理教研室,汕头,515041 4. 汕头大学医学院生物化学与分子生物学教研室,汕头,515041;中国科学院上海生命科学院营养科学研究所,上海,200031 |
| 基金项目： | 国家高技术研究发展计划(863)资助项目(2006AA02A403), 国家自然科学基金资助项目(30370641, 30570829, 30672376), 广东省自然科学基金重点项目(5104541, 7118419)和教育部高等学校博士点重点学科专项基金项目(20050560002, 20050560003) |
| 摘 要： | 以往研究证明，中性粒细胞明胶酶相关载脂蛋白NGAL(neutrophil gelatinase-associated lipocalin)与食管癌密切相关，改变NGAL表达能够对癌细胞的形态结构产生明显影响，但确切的作用机制不明．在酵母细胞中表达NGAL蛋白，柱层析分离纯化．筛选NGALR(NGAL receptor)高表达与弱表达的人食管癌细胞系EC1.71和EC109作为实验细胞模型．5-FAM标记NGAL蛋白，加入到细胞培养上清中，对比研究NGAL蛋白入胞情况、细胞形态学改变、细胞自噬体产生、自噬相关基因表达、细胞内铁离子与铁蛋白水平以及相关细胞信号转导激酶的活性等．结果表明，NGAL蛋白可经由内吞途径进入食管癌细胞发挥作用，致使细胞发生典型的自噬性形态结构变化，自噬体大量产生，自噬相关基因的表达发生相应变化，ERK被激活，但细胞内的铁离子与铁蛋白未受明显影响．上述结果提示，诱导细胞发生自噬是外源性NGAL蛋白经由内吞途径进入食管癌细胞发挥作用的机制之一，与NGAL蛋白跨膜转铁未见直接关联，而ERK信号转导途径可能参与了这一过程． |
| 关 键 词： | NGAL，NGALR，自噬，细胞周期，蛋白质内吞，食管癌细胞 |
| 收稿时间： | 2008/12/17 0:00:00 |
| 修稿时间： | 3/4/2009 12:00:00 AM |
Autophagy induced by NGAL protein in esophageal carcinoma cells |
|
| ZHANG Pi-Xian,FANG Wang-Kai,XU Li-Yan,JIANG Ji-Kai,SHEN Zhong-Ying,DU Ze-Peng,LU Xiao-Feng,ZHOU Fei,XIE Jian-Jun,WU Bing-Li,CUI You-Hong,XIE Dong and LI En-Min.Autophagy induced by NGAL protein in esophageal carcinoma cells[J].Progress In Biochemistry and Biophysics,2009,36(8):978-986. | |
| Authors: | ZHANG Pi-Xian FANG Wang-Kai XU Li-Yan JIANG Ji-Kai SHEN Zhong-Ying DU Ze-Peng LU Xiao-Feng ZHOU Fei XIE Jian-Jun WU Bing-Li CUI You-Hong XIE Dong and LI En-Min |
| Institution: | Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, China;Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, China;Department of Pathology, The Key Immunopathology Laboratory of Guangdong Province,Medical College of Shantou University, Shantou 515041, China;Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, China;Department of Pathology, The Key Immunopathology Laboratory of Guangdong Province,Medical College of Shantou University, Shantou 515041, China;Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, China;Department of Pathology, The Key Immunopathology Laboratory of Guangdong Province,Medical College of Shantou University, Shantou 515041, China;Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, China;Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, China;Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, China;Department of Pathology and Physiology, Medical College of Shantou University, Shantou 515041, China;Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, China;Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, Shanghai 200031, China;Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, China |
| Abstract: | |
| Keywords: | NGAL NGALR autophagy iron protein internalization esophageal carcinoma cells |
| 本文献已被 万方数据 等数据库收录！ | |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 | |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 | |