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| 人原发性肺腺癌转移相关分子的定量蛋白质组学研究 | |
| 引用本文： | 刘迎福,肖志强,张鹏飞,李茂玉,李萃,彭芳,余艳辉,易红,陈主初.人原发性肺腺癌转移相关分子的定量蛋白质组学研究[J].生物化学与生物物理进展,2009,36(4):448-457. |
| 作者姓名： | 刘迎福 肖志强 张鹏飞 李茂玉 李萃 彭芳 余艳辉 易红 陈主初 |
| 作者单位： | 1. 中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室,长沙,410008;中南大学湘雅医学院肿瘤研究所,长沙,410078 2. 中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室,长沙,410008 3. 中南大学湘雅医学院肿瘤研究所,长沙,410078 |
| 基金项目： | 国家重点基础研究发展规划项目(973)(2001CB5120), 教育部跨世纪优秀人才培养计划基金(教计函[2002]48)和湖南省科技厅重大科技专项(04XK1001, 05SK1004-1). |
| 摘 要： | 癌细胞转移是人原发性肺腺癌(lung adenocarcinoma, AdC)死亡率高和预后差的主要原因．为了筛选潜在的肺腺癌转移相关分子标志物，依据临床诊断选取无转移的肺腺癌组织和有转移的肺腺癌组织作为研究对象，首先采用激光捕获显微切割技术(laser capture microdissection, LCM)对两组肺腺癌组织中的癌细胞进行纯化，再利用荧光差异凝胶电泳技术(two-dimensional differential in-gel electrophoresis, 2D-DIGE)分离无转移肺腺癌组和有转移肺腺癌组的癌细胞总蛋白，通过Decyder软件分析两组差异表达的蛋白质点，质谱(mass spectrometry, MS)对差异表达的蛋白质点进行鉴定，Western blot验证部分差异蛋白annexin A1， annexin A2， annexin A3， B23和 S100A9的表达．建立了LCM 纯化的无转移和有转移的肺腺癌组织癌细胞的2D-DIGE图谱，质谱鉴定了20个非冗余差异蛋白质，其中12个蛋白质在有转移肺腺癌组中较无转移肺腺癌组表达上调，8个蛋白质在有转移肺腺癌组中表达下调．Western blot验证分析显示，差异蛋白annexin A1，annexin A2，annexin A3和 S100A9的表达水平在有转移肺腺癌中较无转移肺腺癌增高，B23的表达水平在有转移肺腺癌中较无转移肺腺癌降低．免疫组化进一步证实S100A9在有转移的肺腺癌中较无转移的肺腺癌中表达上调．首次应用LCM技术联合2D-DIGE及MS技术分析鉴定出肺腺癌转移相关蛋白质，为研究肺腺癌的转移分子机制、筛选预测肺腺癌转移的分子标志物奠定了基础． |
| 关 键 词： | 肺腺癌 转移 蛋白质组 激光捕获显微切割技术 荧光差异凝胶电泳技术 |
| 收稿时间： | 2008/8/22 0:00:00 |
| 修稿时间： | 2008/10/21 0:00:00 |
Quantitative Proteomic Analysis of Metastasis-Associated Proteins in Human Primary Lung Adenocarcinoma |
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| LIU Ying-Fu,XIAO Zhi-Qiang,ZHANG Peng-Fei,LI Mao-Yu,LI Cui,PENG Fang,YU Yan-Hui,YI Hong and CHEN Zhu-Chu.Quantitative Proteomic Analysis of Metastasis-Associated Proteins in Human Primary Lung Adenocarcinoma[J].Progress In Biochemistry and Biophysics,2009,36(4):448-457. | |
| Authors: | LIU Ying-Fu XIAO Zhi-Qiang ZHANG Peng-Fei LI Mao-Yu LI Cui PENG Fang YU Yan-Hui YI Hong and CHEN Zhu-Chu |
| Institution: | Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China |
| Abstract: | |
| Keywords: | lung adenocarcinoma metastasis proteome laser capture microdissection two-dimensional differential in-gel electrophoresis |
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