| H9N2亚型禽流感病毒HA基因的克隆及其DNA疫苗的动物免疫试验 |
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| 引用本文： | 何宏轩,秦曦明,张强哲,汤义,刘丽艳,郑昌学,段明星.H9N2亚型禽流感病毒HA基因的克隆及其DNA疫苗的动物免疫试验[J].生物化学与生物物理进展,2004,31(2):163-166. |
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| 作者姓名： | 何宏轩 秦曦明 张强哲 汤义 刘丽艳 郑昌学 段明星 |
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| 作者单位： | 1. 清华大学生物科学与技术系,生物膜与膜生物工程国家重点实验室,北京,100084;河南职业技术师范学院动物科学系,新乡,453003
2. 清华大学生物科学与技术系,生物膜与膜生物工程国家重点实验室,北京,100084 |
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| 摘 要： | 血凝素(HA)是决定禽流感病毒的毒力强弱和免疫原性的主要蛋白质.根据已发表的H9亚型AIV的HA基因序列，设计合成了1对H9 HA特异引物，以AIV A/Chicken/Henan/1/1999/(H9N2)核酸为模板，通过RT-PCR扩增出1条1.6 kb cDNA片段.将HA基因插入pVAX1中，构建了真核表达质粒pVAX-H9.采用活体电击法免疫3周龄SPF鸡10只，剂量为50 μg/只，3周后加强免疫一次，5周后以100倍鸡胚感染剂量(EID)的HA基因同源病毒对所有鸡进行攻毒.其间每周检测抗体水平变化，6周后以棉拭子进行泄殖腔病毒分离.结果为攻毒后免疫组鸡HI效价为9log2～10log2，对照组为2log2～4log2；免疫组病毒分离数为0/10，对照组为10/10.表明所构建的HA基因表达质粒可作为基因疫苗诱导鸡产生免疫保护反应.
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| 关 键 词： | 禽流感病毒，血凝素基因，RT-PCR，克隆，DNA疫苗 |
| 收稿时间： | 2003/6/30 0:00:00 |
| 修稿时间： | 2003年6月30日 |
Cloning of HA Gene of H9N2 Subtype Avian Influenza Virus and Immune Protection Test of Its DNA Vaccine |
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| HE Hong-Xuan,QIN Xi-Ming,ZHANG Qiang-Zhe,TANG Yi,LIU Li-Yan,ZHENG Chang-Xue and DUAN Ming-Xing.Cloning of HA Gene of H9N2 Subtype Avian Influenza Virus and Immune Protection Test of Its DNA Vaccine[J].Progress In Biochemistry and Biophysics,2004,31(2):163-166. |
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| Authors: | HE Hong-Xuan QIN Xi-Ming ZHANG Qiang-Zhe TANG Yi LIU Li-Yan ZHENG Chang-Xue and DUAN Ming-Xing |
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| Institution: | State Key Laboratory of Biomembrane & Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Department of Animal Sciences, Henan Vocational Technique Teachers College, Xinxiang 453003, Chin;State Key Laboratory of Biomembrane & Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;State Key Laboratory of Biomembrane & Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;State Key Laboratory of Biomembrane & Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;State Key Laboratory of Biomembrane & Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Department of Animal Sciences, Henan Vocational Technique Teachers College, Xinxiang 453003, Chin;State Key Laboratory of Biomembrane & Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;State Key Laboratory of Biomembrane & Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China |
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| Abstract: | |
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| Keywords: | avian influenza virus(AIV) HA gene RT PCR cloning DNA vaccine |
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