| 615小鼠血红蛋白α链的氨基酸组成及个别肽段的氨基酸序列 |
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| 引用本文： | 武金霞,张贺迎,王建平,吴经才.615小鼠血红蛋白α链的氨基酸组成及个别肽段的氨基酸序列[J].生物化学与生物物理进展,2001,28(2):259-262. |
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| 作者姓名： | 武金霞 张贺迎 王建平 吴经才 |
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| 作者单位： | 河北大学生命科学学院，保定 071002 |
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| 摘 要： | 用CM-Cellulose-23柱层析分离纯化了615小鼠珠蛋白α链，测定其N端氨基酸残基为缬氨酸.615小鼠珠蛋白α链含有141个氨基酸残基，其中19个亮氨酸残基，10个组氨酸残基，9个缬氨酸残基,上述氨基酸残基的数目与文献中其亲本C57BL不同.用胰蛋白酶水解615小鼠珠蛋白α链，发现有不溶性的‘核心’和可溶性的酶解片段.其中一个酶解肽段从N端数第8位氨基酸残基发生了突变，由亲本的缬氨酸变为亮氨酸.
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| 关 键 词： | 615小鼠，珠蛋白，氨基酸组成，氨基酸序列 |
| 收稿时间： | 2000/2/25 0:00:00 |
| 修稿时间： | 2000年2月25日 |
Amino Acid Composition of
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| WU Jin-Xi,ZhANG He-Ying,WANG Jian-Ping and WU Jing-Cai.Amino Acid Composition of
the[J].Progress In Biochemistry and Biophysics,2001,28(2):259-262. |
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| Authors: | WU Jin-Xi ZhANG He-Ying WANG Jian-Ping and WU Jing-Cai |
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| Institution: | College of life science, Hebei University,Baoding 071002,China;College of life science, Hebei University,Baoding 071002,China;College of life science, Hebei University,Baoding 071002,China;College of life science, Hebei University,Baoding 071002,China |
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| Abstract: | The α chain of hemoglobin of 615 mouse was isolated and purified on CM-Celullose-23 colomn chromatography. The N-terminal amino acid of the α chain was valine determined with DABITC/PITC method.The amino acid composition was determined and it was different from the parent(C57BL)in literature on the number of leucine residue,histine residue and valine residue.An undissoluble ‘core’ and dissoluble peptides were found when the α chain of 615 mouse was hydrolysised by trypsin and it was found that the eighth amino acid residue from N-terminal of one particular peptide fragment mutated from valine (C57BL) to leucine. |
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| Keywords: | 615 mouse hemoglobin amino acid composition amino acid sequence |
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