| 功率超声方法对蛋白质包涵体的解聚 |
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| 引用本文： | 王进,华子春,方勇,唐卫东,朱德煦,冯若,朱昌平,黄金兰,陈兆华.功率超声方法对蛋白质包涵体的解聚[J].生物化学与生物物理进展,1995,22(6):543-545. |
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| 作者姓名： | 王进 华子春 方勇 唐卫东 朱德煦 冯若 朱昌平 黄金兰 陈兆华 |
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| 作者单位： | 南京大学生物化学系,医药生物技术国家重点实验室;南京大学生物化学系,医药生物技术国家重点实验室;南京大学生物化学系,医药生物技术国家重点实验室;南京大学生物化学系,医药生物技术国家重点实验室;南京大学生物化学系,医药生物技术国家重点实验室;南京大学近代声学开放实验室;南京大学近代声学开放实验室;南京大学近代声学开放实验室;南京大学近代声学开放实验室 |
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| 基金项目： | 国家自然科学基金资助项目. |
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| 摘 要： | 用低频功率超声波辐照方法,实现了在低浓度变性剂(4mol/L尿素)中对大肠杆菌体系表达产物缩短的人巨噬细胞集落刺激因子(hM-CSF)包涵体的解聚,以期改进普遍采用的强烈变性剂(8mol/L尿素)溶解包涵体方法,超声处理后的SDS-PAGE在分子量17000上显示了与8mol/L尿素溶解结果相同的条带.表现出功率超声方法在基因工程包涵体解聚中的应用前景.
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| 关 键 词： | 包涵体 功率超声 解聚 |
| 收稿时间： | 1994/12/5 0:00:00 |
| 修稿时间： | 1995/2/28 0:00:00 |
The Dissociation of Protein Inclusion Bodies by High Intensity Ultrasound |
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| Wang Jin,Hua Zichun,Fang Yong,Tang Weidong,Zhu Dexu,Feng Ruo,Zhu Changping,Huang Jinlan and Chen Zhaohua.The Dissociation of Protein Inclusion Bodies by High Intensity Ultrasound[J].Progress In Biochemistry and Biophysics,1995,22(6):543-545. |
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| Authors: | Wang Jin Hua Zichun Fang Yong Tang Weidong Zhu Dexu Feng Ruo Zhu Changping Huang Jinlan and Chen Zhaohua |
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| Institution: | Department of Biochemistry; Nanjing University; National Key Laboratory of Pharmaceutical Biotechnology;Department of Biochemistry; Nanjing University; National Key Laboratory of Pharmaceutical Biotechnology;Department of Biochemistry; Nanjing University; National Key Laboratory of Pharmaceutical Biotechnology;Department of Biochemistry; Nanjing University; National Key Laboratory of Pharmaceutical Biotechnology;Department of Biochemistry; Nanjing University; National Key Laboratory of Pharmaceutical Biotechnology;Modern Acoustics Open Laboratory,Nanjing University;Modern Acoustics Open Laboratory,Nanjing University;Modern Acoustics Open Laboratory,Nanjing University;Modern Acoustics Open Laboratory,Nanjing University |
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| Abstract: | Human macrophage colony stimulating factor(hM-CSF) inclusion bodies expressed in E.coli were dissociated successfully by high intensity ultrasound in a suspension of 4mol/L urea instead of using 8 mol/L urea as solvent.The SDS-PAGE of suspension samples processed by ultrasound shows the same band at 17 000 as that solubilized by 8 mol/L urea.The results show the possibility of developing a new ultrasonic technique for the dissociation of inclusion bodies. |
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| Keywords: | inclusion bodies high intensity ultrasound dissociation |
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