用低频功率超声波辐照方法,实现了在低浓度变性剂(4mol/L尿素)中对大肠杆菌体系表达产物缩短的人巨噬细胞集落刺激因子(hM-CSF)包涵体的解聚,以期改进普遍采用的强烈变性剂(8mol/L尿素)溶解包涵体方法,超声处理后的SDS-PAGE在分子量17000上显示了与8mol/L尿素溶解结果相同的条带.表现出功率超声方法在基因工程包涵体解聚中的应用前景.
Human macrophage colony stimulating factor(hM-CSF) inclusion bodies expressed in E.coli were dissociated successfully by high intensity ultrasound in a suspension of 4mol/L urea instead of using 8 mol/L urea as solvent.The SDS-PAGE of suspension samples processed by ultrasound shows the same band at 17 000 as that solubilized by 8 mol/L urea.The results show the possibility of developing a new ultrasonic technique for the dissociation of inclusion bodies.
王进,华子春,方勇,唐卫东,朱德煦,冯若,朱昌平,黄金兰,陈兆华.功率超声方法对蛋白质包涵体的解聚[J].生物化学与生物物理进展,1995,22(6):543-545
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