| Mn-SOD对CHO细胞电离辐射敏感性的影响 |
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| 引用本文： | 孙娟,陈瑗,周玫,葛忠良,黎明涛.Mn-SOD对CHO细胞电离辐射敏感性的影响[J].生物化学与生物物理进展,1997,24(1):48-52. |
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| 作者姓名： | 孙娟 陈瑗 周玫 葛忠良 黎明涛 |
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| 作者单位： | 第一军医大学自由基医学研究室, 广州 510515;第一军医大学自由基医学研究室, 广州 510515;第一军医大学自由基医学研究室, 广州 510515;军事医学科学院放射医学研究所, 北京 100850;中山医科大学药理教研室, 广州 510089 |
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| 摘 要： | 近年来的研究发现,IL-1和TNF是重要的辐射防护因子,因IL-1和TNF都能选择性诱导Mn-SOD的高度表达,因此认为Mn-SOD可能有辐射防护作用.通过转染有义和反义Mn-SOD cDNA于CHO细胞,进一步说明了Mn-SOD在抗电离辐射损伤中的作用.研究表明,转染有义Mn-SOD cDNA可降低细胞对电离辐射的敏感性, 而转染反义Mn-SOD cDNA的细胞克隆对电离辐射的敏感性升高.
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| 关 键 词： | Mn-SOD 基因转染 电离辐射 |
| 收稿时间： | 1996/2/14 0:00:00 |
| 修稿时间： | 6/6/1996 12:00:00 AM |
Effect of MnSOD on Ionizing Radiation Sensitivity in CHO Cells |
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| SUN Juan,CHEN Yuan,ZHOU Mei,GE Zhongliang and LI Mingtao.Effect of MnSOD on Ionizing Radiation Sensitivity in CHO Cells[J].Progress In Biochemistry and Biophysics,1997,24(1):48-52. |
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| Authors: | SUN Juan CHEN Yuan ZHOU Mei GE Zhongliang and LI Mingtao |
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| Abstract: | IL-1 and TNF have been found to act as protective agents from lethal doses of ionizing radiation in recent years. It was proposed that the mechanisms of the radioprotective effect of IL-1 and TNF may be resulted from to its selective stimulation of manganese supreoxide dismutase(Mn-SOD) expression on both RNA and protein level. Chinese hamster ovary(CHO) cells transfected with sense Mn-SOD cDNA showed decreased radiosensitivity after treatment with X-ray irradiation, whereas clones transfected with anti-sense Mn-SOD cDNA showed increased radiosensitivity. It was demonstrated that overexpression of Mn-SOD could promotes the survival of CHO cells from ionizing radiation. |
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| Keywords: | manganese superoxide dismutase gene transfection ionizing radiation |
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