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人工饲料、转Bt水稻及其亲本稻苗饲养下二化螟种群适合度的比较研究
引用本文:徐艳博,王孟伦,韩兰芝,陈法军.人工饲料、转Bt水稻及其亲本稻苗饲养下二化螟种群适合度的比较研究[J].昆虫知识,2012,49(6):1535-1540.
作者姓名:徐艳博  王孟伦  韩兰芝  陈法军
作者单位:1. 南京农业大学植物保护学院昆虫系昆虫信息生态研究室 南京210095
2. 南京农业大学食品科技学院 南京210095
3. 中国农业科学院植物保护研究所 北京 100193
基金项目:国家基础发展规划“973”项目(2010CB126200)、国家公益性行业(农业)科研专项项目(200903051)、转基因生物新品种培育重大专项课题(2011ZX08012-005和2012ZX08011002)、国家自然科学基金(31272051)和教育部霍英东基金“全球气候变化下转Bt作物氮肥优化管理及其害虫控制技术研究”(122033).
摘    要:以人工饲料、转Bt水稻"克螟稻"(cry1Ab纯和基因型)及其对照亲本"秀水11"稻苗为供试寄主植物开展二化螟Chilo suppressalis(Walker)1~5龄幼虫的室内饲养试验,以明确不同龄期二化螟种群的生活史参数。试验结果表明:二化螟在低龄时死亡率最高。克螟稻对二化螟各个龄期表现出高抗性,其各个龄期在克螟稻上均不能化蛹,随着龄期的增加二化螟的耐受性增强。以秀水11和人工饲料饲养二化螟对其蛹期、成虫期、单雌产卵量、羽化率的影响无显著性差异,以人工饲料饲养的二化螟蛹重显著高于以秀水11饲养的二化螟的蛹重,蛹重与人工饲料饲养时间呈正相关。与秀水11幼苗相比,人工饲料饲养下有利于二化螟雌虫的分化。

关 键 词:二化螟  人工饲料  转Bt水稻  常规水稻  生活史参数

The suitability of Chilo suppressalis reared on artificial diet, seedings of transgenic Bt rice and nontransgenic rice
XU Yan-Bo WANG Meng-Lun,HAN Lan-Zhia CHEN Fa-Jun.The suitability of Chilo suppressalis reared on artificial diet, seedings of transgenic Bt rice and nontransgenic rice[J].Entomological Knowledge,2012,49(6):1535-1540.
Authors:XU Yan-Bo WANG Meng-Lun  HAN Lan-Zhia CHEN Fa-Jun
Institution:** (1. Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China; 2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 3. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
Abstract:The effect of rearing of the 1st to 5th instar larvae of the rice stem borer, Chilo suppressalis ( Walker), on artificial diet, seedlings of transgenic Bt rice with pure crylAb (cv. KMD), and the parent line nontransgenic rice (cv. XSDll), were assessed and compared. The results showed that the nymphs at lower instar stages had high mortality. Moreover, transgenic Bt rice was highly resistant to all instar larvae, especially the younger instar individuals. Moreover, there were no significant differences in the duration of the pupal or adult stages, fecundity and molting rates of larvae reared on XSDI1 and the artificial feed. Furthermore, the pupal weight as larvae fed on the artificial diet was significantly higher than that as fed on nontransgenic rice, and larvae fed the artificial diet displayed a positive relationship between pupal weight and rearing time. Compared to nontransgenic rice, artificial feeds were more beneficial and advantageous for the differentiation of C. suppressalis females.
Keywords:Chilo suppressalis  artificial feed  transgenic bt rice  nontrsansgenic rice  life-history indexes
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