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| 利用正交法确定测定意大利蜜蜂幼虫细胞色素P4500-脱乙基活性最佳反应条件 | |
| 引用本文： | 唐晓伟,高希武,刁青云.利用正交法确定测定意大利蜜蜂幼虫细胞色素P4500-脱乙基活性最佳反应条件[J].昆虫知识,2011,48(5):1513-1518. |
| 作者姓名： | 唐晓伟 高希武 刁青云 |
| 作者单位： | 1. 中国农业科学院蜜蜂研究所 北京100093;中国农业大学昆虫学系 北京100193 2. 中国农业大学昆虫学系 北京100193 3. 中国农业科学院蜜蜂研究所 北京100093 |
| 基金项目： | 国家科技支撑计划课题(2006BAD06B04) |
| 摘 要： | 荧光分光光度法检测意大利蜜蜂Apis mellifera ligustica Spinal细胞色素P450 O-脱乙基活性方便快捷、较灵敏,以7-乙氧基香豆素为底物.通过测定其产物7-羟基香豆素的荧光变化来确定细胞色素P450 O-脱乙基的活性.在利用荧光分光光度法检测意大利蜜蜂幼虫细胞色素P450 O-脱乙基活性过程... |
| 关 键 词： | 意大利蜜蜂 细胞色素P450 O-脱乙基活性 正交试验 荧光分光光度法 最佳条件 |
The optimum conditions for assaying 7-ethoxycoumarin O-deethoxylation of cytochrome P450 from the Italian honeybee(Apis mellifera ligustica Spinol) by orthogonal matrix method |
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| TANG Xiao-Wei,GAO Xi-Wu,DIAO Qing-Yun.The optimum conditions for assaying 7-ethoxycoumarin O-deethoxylation of cytochrome P450 from the Italian honeybee(Apis mellifera ligustica Spinol) by orthogonal matrix method[J].Entomological Knowledge,2011,48(5):1513-1518. | |
| Authors: | TANG Xiao-Wei GAO Xi-Wu DIAO Qing-Yun |
| Institution: | TANG Xiao-Wei~1,2** GAO Xi-Wu~2 DIAO Qing-Yun~1***(1.Institute of Apicultural Research,Chinese Academy of Agricultural Science,Beijing 100093,China,2.Department of Entomology,China Agricultural University,Beijing 100193,China) |
| Abstract: | The 7-ethoxycoumarin O-deethoxylation(ECOD) activity of cytochrome P450(CytP450) was determined using fluorescence spectrophotometry with 7-ethoxycoumarin as a substrate.This method was sensitive and convenient.The optimal conditions for assaying the ECOD activity of cytochrome P450(CytP450) in Apis mellifera ligustica Spinol were determined using the orthogonal matrix method.The results show that reaction time was the most important factor determining the ECOD activity of CytP450 in honeybees.The relative ... |
| Keywords: | Apis mellifera ligustica O-deethoxylation cytochrome P450 monooxygenase orthogonal experiment fluorescence spectrophotometry optimal conditions |
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