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龙葵上柑橘木虱存活观察与黄龙病检测
引用本文:卢慧林,欧阳革成,方小端.龙葵上柑橘木虱存活观察与黄龙病检测[J].环境昆虫学报,2015,37(3):543-547.
作者姓名:卢慧林  欧阳革成  方小端
作者单位:(1. 贵州大学昆虫研究所,贵州山地农业病虫害重点实验室,贵阳 550025;2.贵州轻工职业技术学院,贵阳550000;3. 贵州检验检疫局,贵阳550000;4. 中国科学院动物研究所,动物进化与系统学重点实验室,北京 100101;5. 北京林业大学省部共建森林培育与保护教育部重点实验室,北京 100083)
基金项目:贵州省科学技术基金(黔科合J字\[2013\]2149号);国家质检总局重点科研项目(2014Ik022)
摘    要:柑橘木虱Diaphorina citri是毁灭性病害黄龙病的媒介,其寄主范围比较严格,仅为芸香科内的一些植物。研究发现,在自然状态下,柑橘木虱成虫可在柑橘园中常见茄科Solanaceae杂草-龙葵Solanum nigrum上停留。对比试验显示,木虱成虫在龙葵上的存活期最长可达45 d,而在假臭草Eupatorium catarium、含水海绵和无水海绵上分别为24 d、9 d和2 d;通过实时荧光PCR检测发现部分龙葵叶片中含有黄龙病病原菌。这些非寄主植物可能有助于柑橘木虱躲避不良环境或长距离迁移扩散,成为柑橘木虱和黄龙病的潜在库源。

关 键 词:桔小实蝇    适生性    CLIMEX    ArcGIS    贵州

Survival observation of Diaphorina citri and detection of Huanglongbing on Solanum nigrum
LU Hui-Lin,OUYANG Ge-Cheng,FANG Xiao-Duan.Survival observation of Diaphorina citri and detection of Huanglongbing on Solanum nigrum[J].Journal of Environmental Entomology,2015,37(3):543-547.
Authors:LU Hui-Lin  OUYANG Ge-Cheng  FANG Xiao-Duan
Institution:(Guangdong Entomological Institute,Guangdong Public Laboratory of Wild Animal Conservation and Utilization,Guangdong Key Laboratory of Integrated Pest Management in Agriculture,Guangzhou 510260, China)
Abstract:Citrus psyllid is vector of huanglongbing, the host range of which is only part of Rutaceae plants. Under natural condition, we found that psyllid adults could stay on the Solanum nigrum, Solanaceae. Contrasting experiments showed that the longest survival time of psyllid adults on S. Nigrum was 45 days, and that on Eupatorium catarium, sponge with water and without water was 24 days, 9 days and 2 days respectively. Based on the Q-PCR analysis, huanglongbing has been detected on the leaves of S. Nigrum. These non-host plants might help psyllid survival in detrimental conditions and relocation diffusion for long distance, and become potential source of psyllid and huanglongbing.
Keywords:Bactrocera dorsalis  potencial geographical distribution  CLIMEX  ArcGIS  Guizhou
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