| 铵盐和硝酸盐稳定同位素丰度测定方法及其应用案例 |
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| 引用本文： | 刘冬伟,图影,方运霆.铵盐和硝酸盐稳定同位素丰度测定方法及其应用案例[J].生态学杂志,2017,28(7):2353-2360. |
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| 作者姓名： | 刘冬伟 图影 方运霆 |
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| 作者单位： | 1.中国科学院沈阳应用生态研究所森林生态与管理重点实验室, 沈阳 110016;2.中国科学院大学, 北京 100049;3.中国科学院清原森林生态系统观测研究站, 沈阳 110016 |
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| 基金项目： | 本文由国家重点研发计划项目(2016YFA0600802)、中国科学院战略性先导科技专项(B类;XDB15020200)、国家自然科学基金项目(31422009,31600358)和中国科学院前沿科学重点研究项目(QYZDB-SSW-DQC002)资助 |
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| 摘 要： | 综述了过去几十年来铵盐和硝酸盐稳定同位素丰度测定方法的历史发展变化,分析了各种方法的优缺点,并对新方法作了介绍和推荐.目前铵盐稳定同位素丰度的最新测定方法为次溴酸盐氧化结合羟胺还原法,硝酸盐氮氧同位素丰度主流的测定方法为反硝化细菌法和镉粉叠氮酸还原化学法.这些方法的主要共同特点是以N2O为分析物,分析精度高,对样品的含氮量需求小,一般只需要10～60 nmol N,适用于低浓度样品.新方法的建立对于国内外开展氮素循环研究将起到极大的推动作用.
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| 关 键 词： | 铵盐 硝酸盐 同位素组成 |
| 收稿时间： | 2017-06-19 |
Isotope analysis of ammonium and nitrate: A review on measured methods and their application |
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| LIU Dong-wei,TU Ying,FANG Yun-ting.Isotope analysis of ammonium and nitrate: A review on measured methods and their application[J].Chinese Journal of Ecology,2017,28(7):2353-2360. |
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| Authors: | LIU Dong-wei TU Ying FANG Yun-ting |
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| Institution: | 1.Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Qingyuan Forest CERN, Chinese Academy of Sciences, Shenyang 110016, China |
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| Abstract: | In the past several decades, a variety of methods have been developed for measuring the isotopic composition of ammonium (δ15N) and nitrate (δ15N and δ18O). This review summarized the advantages and disadvantages of these methods. Nowadays, the most popular method for measu-ring δ15N of ammonium is the combined hypobromite (BrO-) and hydroxylamine (NH2OH) me-thod, while for δ15N and δ18O of nitrate is the denitrifier method and the sodium azide (NaN3) me-thod. These methods convert NH4+ or NO3- into nitrous oxide (N2O) and measure its isotopic compositions, with higher analytical precision because of the lower background concentration of atmospheric N2O. Accordingly, these methods are suitable for the samples with lower N concentration, and normally require 10-60 nmol N. The development of new methods for measuring N isotopic composition has greatly stimulated the studies in nitrogen cycling worldwide. |
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| Keywords: | ammonium nitrate isotopic composition |
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