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象山港典型生境沉积物反硝化细菌群落丰度与结构多样性
引用本文:修玉娜,陈松泽,李雪英,姜亚洲,唐保军,平仙隐,范瑞良,李楠楠,全为民.象山港典型生境沉积物反硝化细菌群落丰度与结构多样性[J].生态学杂志,2021(1):84-92.
作者姓名:修玉娜  陈松泽  李雪英  姜亚洲  唐保军  平仙隐  范瑞良  李楠楠  全为民
作者单位:中国水产科学研究院东海水产研究所农业农村部东海渔业资源开发重点实验室;上海海洋大学水产与生命学院;同济大学海洋地质国家重点实验室
基金项目:上海市自然科学基金项目(19ZR1469900);中央公益性科研院所基本科研业务费项目(L32201921880)资助。
摘    要:通过高通量测序和qPCR技术对象山港4种典型生境(牡蛎养殖区OA、海带养殖区SA、自然岛礁区NR、人工鱼礁区AR)和对照区CK的沉积物反硝化细菌丰度和群落结构进行了测定分析,并探讨了反硝化细菌群落与环境因子之间的相关关系。结果表明:沉积物nirK型反硝化细菌丰度在5种生境间没有显著性差异,而沉积物nirS型反硝化细菌丰度排序为AR
关 键 词:NIRK  nirS  高能量测序  定量PCR    生物多样性

Abundance and structure diversity of denitrifying bacterial community in sediment of the typical habitats of Xiangshan Bay
XIU Yu-na,CHEN Song-ze,LI Xue-ying,JIANG Ya-zhou,TANG Bao-jun,PING Xian-yin,FAN Rui-liang,LI Nan-nan,QUAN Wei-min.Abundance and structure diversity of denitrifying bacterial community in sediment of the typical habitats of Xiangshan Bay[J].Chinese Journal of Ecology,2021(1):84-92.
Authors:XIU Yu-na  CHEN Song-ze  LI Xue-ying  JIANG Ya-zhou  TANG Bao-jun  PING Xian-yin  FAN Rui-liang  LI Nan-nan  QUAN Wei-min
Institution:(Ministry of Agriculture and Rural Affairs Key Laboratory of East China Sea Fishery Resources Exploitation,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;College of Fishery and Life Science,Shanghai Ocean University,Shanghai 201300,China;State Key Laboratory of Marine Geology,Tongji University,Shanghai 200092,China)
Abstract:We measured the abundance and community structure of denitrifying bacteria in sedi-ments in four typical habitats(oyster aquaculture area(OA),seagrass aquaculture area(SA),natural island reef(NR),artificial reef(AR))and control habitat(CK,a natural sediment-laden harbor site)of Xiangshan Bay using high-throughput sequencing and qPCR techniques.We further analyzed the correlations between denitrifying bacterial communities and abiotic factors.The results showed that the abundances of nirK-type denitrifying bacteria didn’t differ among those five habitats and that the abundance of nirS-type denitrifying bacteria was the lowest in the AR habitat.The abundance order of nirS-type denitrifying bacteria in sediments was AR
Keywords:nirK  nirS  high-throughput sequencing  qPCR  nitrogen  biodiversity
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