|
|||||
|
|
| 葛根总黄酮提取工艺的研究 | |
| 引用本文： | 刘火安,王伯初,贾云,戴传云,王宾豪.葛根总黄酮提取工艺的研究[J].生物技术通讯,2006,17(1):43-45. |
| 作者姓名： | 刘火安 王伯初 贾云 戴传云 王宾豪 |
| 作者单位： | 1. 重庆大学生物工程学院,重庆,400044;重庆科技学院化学与生物工程学院,重庆,400050 2. 重庆大学生物工程学院,重庆,400044 3. 重庆科技学院化学与生物工程学院,重庆,400050 |
| 基金项目： | 重庆市自然科学基金项目(CSTC.2004BB5122) |
| 摘 要： | 目的:筛选提取葛根总黄酮的最佳工艺条件。方法:采用单因素和正交试验对提取葛根总黄酮的工艺条件进行优化;利用紫外可见分光光度计测量葛根总黄酮的含量;利用高效液相色谱仪测量葛根素的含量;分别以葛根素和葛根总黄酮的含量为指标进行最优工艺的评价。结果:最佳工艺条件是以9倍量的30%乙醇提取3次,每次1h。结论:该提取工艺简单,成本低,适合于生产应用。 |
| 关 键 词： | 葛根 总黄酮 葛根素 提取工艺 优化 |
| 文章编号： | 1009-0002（2006）01-0043-03 |
| 收稿时间： | 2005-06-24 |
| 修稿时间： | 2005年6月24日 |
Study on the Process for Extraction of Total Flavonoids from Radix of Pueraria |
|
| LIU Huo-an,WANG Bo-chu,JIA Yun,DAI Chuan-yun,WANG Bin-hao.Study on the Process for Extraction of Total Flavonoids from Radix of Pueraria[J].Letters in Biotechnology,2006,17(1):43-45. | |
| Authors: | LIU Huo-an WANG Bo-chu JIA Yun DAI Chuan-yun WANG Bin-hao |
| Abstract: | Objective To optimize the extracting process for total flavonoids from radix Puerariae. Methods Single factor and orthogonal design were respectively utilized to optimize extracting process of radix Puerariae. Concentration of total flavonoids was measured by the UV spectrophotometer and concentration of puerarin was measured by HPLC. The content of puerarin and the total flavonoids were used as indexes to evaluate the optimal extracting conditions. Results The results were showed that the optimum extraction process were as follows 30% alcohol as solvent 9 times of alcohol amount 3 times of extraction and 1 h per time. Conclusion This process was simple and feasible with good effect of extraction which can meet with the industrial requirements. |
| Keywords: | radix of Pueraria total flavonoids puerarin optimization extracting process |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |