| 培养基对重组质粒pcDNA3—HGF产率的影响 |
| |
| 引用本文： | 毕建进,张庆林,魏汉东,吴祖泽.培养基对重组质粒pcDNA3—HGF产率的影响[J].生物技术通讯,2000,11(4):271-271. |
| |
| 作者姓名： | 毕建进 张庆林 魏汉东 吴祖泽 |
| |
| 作者单位： | 北京放射医学研究所,北京,100850 |
| |
| 摘 要： | 重组质粒ＤＮＡ用于基因治疗需要大量符合药学规格的质粒ＤＮＡ，发酵是影响质粒产率的关键步骤之一。比较了４种不同的培养基对ｐｃＤＮＡ３－ＨＧＦ产率的影响，其中自配复合培养基（ＺＰ）质粒产率最高，而ＬＢ培养基的质粒产率最低，５Ｌ发酵罐发酵最终ｐｃＤＮＡ３－ＨＧＦ产率达７２ｍｇ／Ｌ，为大规模发酵生产提供依据。
|
| 关 键 词： | 发酵 pcDNA3－HGF 培养基 产率 重组质粒 |
Effect of growth medium on the yield of recombinant plasmid pcDNA 3HGF |
| |
| Bi Jianjin,Zhang Qinglin,Wei Handong,Wu Zuze.Effect of growth medium on the yield of recombinant plasmid pcDNA 3HGF[J].Letters in Biotechnology,2000,11(4):271-271. |
| |
| Authors: | Bi Jianjin Zhang Qinglin Wei Handong Wu Zuze |
| |
| Abstract: | Four growth medium(LB-TB-XL-ZP)were used to assess their effect on the yield of recombinant plasmid pcDNA3-HGF for plasmid-basedsene therapy. ZP midium containing more glucose was found to have highest plasmid yield.The yield of 5 L fermentation produced a yield of 72 mg/L of pcDNA3-HGF. |
| |
| Keywords: | fermentation pcDNA3-HGF growth medium yield |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
