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| 驱虫斑鸠菊对人黑素瘤细胞增殖酪氨酸酶及黑素生成影响的研究 | |
| 引用本文： | 邓瑞春,周勇,章金刚,徐建国,尚靖,于鲁海.驱虫斑鸠菊对人黑素瘤细胞增殖酪氨酸酶及黑素生成影响的研究[J].生物技术通讯,2002,13(2):135-137. |
| 作者姓名： | 邓瑞春 周勇 章金刚 徐建国 尚靖 于鲁海 |
| 作者单位： | 1. 北京中医药大学中西医结合研究所,北京100029 2. 军事医学科学院野战输血研究所 3. 新疆自治区临床药学研究所 |
| 摘 要： | 探讨驱虫斑鸠菊对A375人黑素瘤细胞株细胞增殖、黑素合成以及细胞内酪氨酸酶的作用。四甲基偶氮唑蓝（MTT）比色法测定药物对细胞增殖的影响；采用酶学方法研究药物对酪氨酸酶活性的影响；475nm比色法测定黑素含量。结果表明驱虫斑鸠菊可以增强A375人黑素瘤细胞增殖，提高酪氨酸酶和黑色素合成能力。说明驱虫斑鸠菊治疗白癜风是通过增强酪氨酸酶活性及促进黑素合成而发挥作用的。 |
| 关 键 词： | 驱虫斑鸠菊 酪氨酸酶 人黑素瘤细胞 白癜风 黑素生成 细胞增殖 |
| 文章编号： | 1009-0002(2002)02-0135-03 |
| 修稿时间： | 2001年11月20 |
Effect of Vernoniaanthelmintica willd on tyrosinase activity and melanogenesis in A375 human melanoma |
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| DENG Rui-chun,ZHOU Yong,ZHANG Jin-gang,XU Jian-guo,SHANG Jing,YU Lu-hai.Effect of Vernoniaanthelmintica willd on tyrosinase activity and melanogenesis in A375 human melanoma[J].Letters in Biotechnology,2002,13(2):135-137. | |
| Authors: | DENG Rui-chun ZHOU Yong ZHANG Jin-gang XU Jian-guo SHANG Jing YU Lu-hai |
| Abstract: | To study the effect of Vernoniaanthelmintica willd,on cell proliferation tyrosinase activity and the melanogenesis of A375human melanoma cell.Tyrosinase activity was measured by zymologic method with its absorption at475nm.MTT was used to study the proliferation of A375human melanoma cell.The melanin was detected by its absorption at475nm.Vernoniaanthelmintica willd enhanced significantly the synthesis of tyrosinase,cell proliferation and the melanogenesis of A375human melanoma cell in vitro.Effect of Vernoniaanthelmintica willd treating vitiligo is relative with enhancing activity of tyrosinase and melanogenesis |
| Keywords: | Vernoniaanthelmintica willd tyrosinase vitiligo human melanoma |
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