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| 致病性猪链球菌2型溶血素基因的克隆与表达 | |
| 引用本文： | 梁毅,郭桂英,李惠军,栗桂玲,郑继平,张兆山.致病性猪链球菌2型溶血素基因的克隆与表达[J].生物技术通讯,2009,20(3):367-369. |
| 作者姓名： | 梁毅 郭桂英 李惠军 栗桂玲 郑继平 张兆山 |
| 作者单位： | 1. 海南大学,农学院,海南,海口,570228 2. 海南大学,教务处,海南,海口,570228 3. 海南大学,热带生物资源教育部重点实验室,海南,海口,570228;海南大学,农学院,海南,海口,570228 4. 军事医学科学院,生物工程研究所,北京,100071 |
| 基金项目： | 教育部科学技术研究重点项目，海南省自然科学基金，海口市农业三项基金，海南省教育厅高等学校科研项目 |
| 摘 要： | 目的：克隆溶血素基因,为评价溶血素的毒力与抗原活性,构建猪链球菌疫苗提供依据。方法：从致病性猪链球菌II型四川资阳分离株基因组中分离溶血素基因,经中间T载体,将其克隆至改造的pGEX-6p1中,最后通过蛋白质电泳和溶血实验检验溶血素的表达及活性。结果：SDS-PAGE结果表明,重组溶血素在5h表达水平最高,10h次之,15h最弱;溶血实验重组菌周围形成明显的透明溶血环。结论：溶血素为分泌性早期表达,具有正常的β溶血活性。 |
| 关 键 词： | 致病性猪链球菌2型 溶血素 克隆 表达 |
Clone and Expression of Sly Gene from Streptococcus suis Serotype 2 |
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| LIANG Yi,GUO Gui-Ying,LI Hui-Jun,LI Gui-Ling,ZHENG Ji-Ping,ZHANG Zhao-Shan.Clone and Expression of Sly Gene from Streptococcus suis Serotype 2[J].Letters in Biotechnology,2009,20(3):367-369. | |
| Authors: | LIANG Yi GUO Gui-Ying LI Hui-Jun LI Gui-Ling ZHENG Ji-Ping ZHANG Zhao-Shan |
| Institution: | (. a. Key Laboratory of Tropical Biological Resources, Ministry of Education; b. Department of Biological Technology; c Academic Affairs Office; Hainan University, Haikou 570225; 2. Beijing Institute of Biotechnology, Beijing 100071; China) |
| Abstract: | Objective： To clone and evaluate the virulence and protection of suilysin（SLY）, for developing a vaccine against the infective Streptococcus suis type 2（SS2）. Methods： Sly gene was amplified from a SS2 strain isolated in Ziyang, Sichuan, and ligased into T-vector, then transferred into a refined pGEX-6pl without Laclq gene. Protein SDS-PAGE electrophoresis and hemolysis test was used to check the expression and activity of SLY. Results： The SLY expression of recombinant strain was lower by degree for growth at 5 h, 10 h and 15 h, and transparent ring around recombinant strain were observed in electrophoresis and hemolysis test respectively. Conclusion： Secreted SLY was expressed in early stage and exhibited a distinctive β-hemolysis activity. |
| Keywords: | infectious Streptococcus suis type 2 hemolysin clone expression |
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