| 鼠疫耶尔森氏菌VcrV基因在大肠杆菌中的高效表达 |
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| 引用本文： | 王忠泽,荫俊,侯晓军,宋伟,张松乐,王威,白洁.鼠疫耶尔森氏菌VcrV基因在大肠杆菌中的高效表达[J].生物技术通讯,2001,12(2):96-98. |
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| 作者姓名： | 王忠泽 荫俊 侯晓军 宋伟 张松乐 王威 白洁 |
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| 作者单位： | 1. 军事医学科学院微生物流行病研究所
2. 白求恩医科大学 |
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| 摘 要： | 将测序后的鼠疫耶尔森氏菌（Yersinia pestis)LcrV基因重组质粒pGEM－T／ypV酶切，克隆于原核表达载体pBV220，构建成pBV/ypV表达质粒，转化大肠杆菌DH5α，进行PCR及酶切鉴定，筛选阳性克隆，进行温控诱导表达，SDS－PAGE检测表达产物，在相对分子质量38000处有－表达条带，经薄层扫描分析目的蛋白带占全菌蛋白的38．4％以上，主要以可溶形式存在。
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| 关 键 词： | 鼠疫耶尔森氏菌 基因表达 VcrV基因 |
| 修稿时间： | 2000年8月4日 |
High-level expression of Yersinia pestis LcrV gene in Escherichia coli |
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| WANG Zhong-Ze,YIN Jun,HOU Xiao-Jun,SONG Wei,ZHANG Song-le,WANG Wei,BAI Jie.High-level expression of Yersinia pestis LcrV gene in Escherichia coli[J].Letters in Biotechnology,2001,12(2):96-98. |
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| Authors: | WANG Zhong-Ze YIN Jun HOU Xiao-Jun SONG Wei ZHANG Song-le WANG Wei BAI Jie |
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| Abstract: | The recombinant expression plasmid pBV/ypV was constructed by inserting the DNA fragment of Yersinia pestis LcrV gene into pBV220 and transformed into E.coli DH5α cell. The positive clones were screened out by PCR and enzymes digesting, and then through temperature regulated inducement expression, the expressed product was detected by SDS-PAGE, and an expression band about 38000 was found. The aim protein representing 38.4% of total cell protein and the most in soluble form. |
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| Keywords: | Yersinia pestis clone gene expression LcrV gene |
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